A0A433SJ57 · A0A433SJ57_ELYCH
- ProteinTranslation factor GUF1 homolog, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids754 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
Catalytic activity
- 5-taurinomethyluridine34 in tRNA + AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] = 5-taurinomethyl-2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
RHEA-COMP:11732 CHEBI:87172 Position: 34+ CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 = RHEA-COMP:11733 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrial matrix | |
Molecular Function | ATP binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | ribosome binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-5-taurinomethyluridine 2-sulfurtransferase | |
Biological Process | positive regulation of translation | |
Biological Process | translation | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranslation factor GUF1 homolog, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Gastropoda > Heterobranchia > Euthyneura > Panpulmonata > Sacoglossa > Placobranchoidea > Plakobranchidae > Elysia
Accessions
- Primary accessionA0A433SJ57
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-149 | Tr-type G | ||||
Sequence: MKEQVLDSMDIERERGITIKAQSVTLDYHAKDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQTVANCYTAIEQDLEVIPILNKIDLPSADPEKVCEEIEEIIGIDASDATTCSAKTGVGVIDVLETIVRKVPPPE | ||||||
Domain | 526-579 | Rhodanese | ||||
Sequence: KKLLEKQKEEKIIVGISGGVDSSVSALLLKQQGYDVVGVFMKNWEEDDNDEFCS |
Sequence similarities
Belongs to the GTP-binding elongation factor family. LepA subfamily.
Belongs to the MnmA/TRMU family.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length754
- Mass (Da)82,996
- Last updated2019-05-08 v1
- ChecksumDF1775D04120A0E3
Keywords
- Technical term