A0A432GQJ8 · A0A432GQJ8_9DELT
- ProteinGlyceraldehyde-3-phosphate dehydrogenase
- Genegap
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids336 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
Catalytic activity
- D-glyceraldehyde 3-phosphate + phosphate + NAD+ = (2R)-3-phospho-glyceroyl phosphate + NADH + H+
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-13 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 34 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 78 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 120 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 152-154 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Active site | 153 | Nucleophile | |||
Site | 180 | Activates thiol group during catalysis | |||
Binding site | 183 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 212-213 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 235 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 317 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | NAD binding | |
Molecular Function | NADP binding | |
Molecular Function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor | |
Biological Process | glucose metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlyceraldehyde-3-phosphate dehydrogenase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Deltaproteobacteria > SAR324 cluster
Accessions
- Primary accessionA0A432GQJ8
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-153 | Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding | |||
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length336
- Mass (Da)36,093
- Last updated2019-05-08 v1
- ChecksumC1FA54E2ED5990A1
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QNZM01000062 EMBL· GenBank· DDBJ | RTZ82057.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QNZK01000140 EMBL· GenBank· DDBJ | RTZ85826.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QNZH01000148 EMBL· GenBank· DDBJ | RTZ90114.1 EMBL· GenBank· DDBJ | Genomic DNA |