A0A432F810 · A0A432F810_UNCVE
- ProteinSerine hydroxymethyltransferase
- GenerpiB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids724 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
Cofactor
Pathway
Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 9 | Nucleophile | |||
Active site | 15 | ||||
Active site | 124 | Proton donor | |||
Binding site | 430 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 434-436 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 538 | Plays an important role in substrate specificity | |||
Binding site | 662-664 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | cobalt ion binding | |
Molecular Function | glycine hydroxymethyltransferase activity | |
Molecular Function | intramolecular oxidoreductase activity, interconverting aldoses and ketoses | |
Molecular Function | protein tyrosine phosphatase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | serine binding | |
Molecular Function | zinc ion binding | |
Biological Process | carbohydrate metabolic process | |
Biological Process | folic acid metabolic process | |
Biological Process | glycine biosynthetic process from serine | |
Biological Process | L-serine catabolic process | |
Biological Process | protein dephosphorylation | |
Biological Process | tetrahydrofolate interconversion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine hydroxymethyltransferase
- EC number
- Short namesSHMT ; Serine methylase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Verrucomicrobiota
Accessions
- Primary accessionA0A432F810
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 539 | N6-(pyridoxal phosphate)lysine | |||
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-150 | Phosphotyrosine protein phosphatase I | |||
Sequence similarities
Belongs to the LacAB/RpiB family.
Belongs to the SHMT family.
Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length724
- Mass (Da)77,757
- Last updated2019-05-08 v1
- Checksum8949679EB04198A7
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QOAX01000174 EMBL· GenBank· DDBJ | RTZ67544.1 EMBL· GenBank· DDBJ | Genomic DNA |