A0A432ELL5 · A0A432ELL5_9GAMM

Function

function

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.
Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.

Features

Showing features for site, binding site, active site.

Type
IDPosition(s)Description
Site44Part of a proton relay during catalysis
Binding site45pyruvate (UniProtKB | ChEBI)
Site49L-lysine inhibitor binding; via carbonyl oxygen
Site80L-lysine inhibitor binding
Site84L-lysine inhibitor binding
Site106L-lysine inhibitor binding
Site107Part of a proton relay during catalysis
Active site133Proton donor/acceptor
Active site161Schiff-base intermediate with substrate
Binding site203pyruvate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4-hydroxy-tetrahydrodipicolinate synthase activity
Biological Processdiaminopimelate biosynthetic process
Biological Processlysine biosynthetic process via diaminopimelate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-tetrahydrodipicolinate synthase
  • EC number
  • Short names
    HTPA synthase

Gene names

    • Name
      dapA
    • ORF names
      DSZ33_04155

Organism names

Accessions

  • Primary accession
    A0A432ELL5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer; dimer of dimers.

Family & Domains

Sequence similarities

Belongs to the DapA family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    290
  • Mass (Da)
    31,357
  • Last updated
    2019-05-08 v1
  • Checksum
    97E55360D6982188
MFHGSMVALVTPMHADGSLNFTALEKLVEFHVESGTDAIVSMGTTGESATLNEKEHIEVIRRTVKAVAGRIPVIAGTGANSTTEAITLTRQAREVDADACLLVTPYYNKPTQEGLYLHFRAVAEAVDIPQILYNVPGRTAVDMSAETTARLSELDGVIGIKDATGDMQRAEELKELCKTGFEMYSGDDATTLDFIERGGQGCISVTANVAPVKMHEMCKLALAGDRAGAERINEDLKLLHERLFLESSPIPVKWAVARLGLMPDGIRLPLTPLSAQYQDDVLEAMQRAGV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QOAV01000139
EMBL· GenBank· DDBJ
RTZ59589.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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