A0A426W1R6 · A0A426W1R6_9BACT

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site191-192ATP (UniProtKB | ChEBI)
Binding site273ATP (UniProtKB | ChEBI)
Binding site295ATP (UniProtKB | ChEBI)
Binding site304ATP (UniProtKB | ChEBI)
Active site459Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      P794_01885

Organism names

Accessions

  • Primary accession
    A0A426W1R6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain68-173THUMP
Domain412-484Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    487
  • Mass (Da)
    54,835
  • Last updated
    2019-05-08 v1
  • Checksum
    8D4D80A6DF75AFFF
MESPSVNTQKFILKLFPEIMVKGSSAKRQMIGQLYNNLIKLLNTYSPEIKVRKFSDKIEVVSPTNVLTEVRQTLLHTPGIEQVLEALQFDNMDELSDIKVKVSEIMAKTVEGKTFVVRAKRTGTHPFNSTVLEQTVGGYMLAHCNAKGVDLHHPEVTVRIELINKQLNIITTKYAGLGGFPIGTQGDILSLMSGGFDSTVASYLTMKRGIKTHFIFFNLGGIAHEIGVKQVALYLWSKYGASHRVSFISVPFDKVIEEIFRSTHETYMGVTLKRLMLMAAEKIANTMEIDALVTGESVAQVSSQTLRNLALIDQVTNKLVLRPLATMNKPDIINIANEIGTRRFAENMPEYCGVISKNPITHGSYKRMEKEAKRFDYAVLDKAAEEAVMIYVDEIIDDVSNAAPIEVIHDLSKGEYVVIDIRPEEECLSTSCKSIKIPFHKLKTEFKKLPQDKEYLLYCEKGIMSQLHAQYLRDSESCENVRVYRPQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JQIX01000015
EMBL· GenBank· DDBJ
RRS32151.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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