A0A424Z0B6 · A0A424Z0B6_9BACT
- ProteinCarbamoyl phosphate synthase small chain
- GenecarA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
Catalytic activity
- hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H+
- L-glutamine + H2O = L-glutamate + NH4+
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 45 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 240 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 242 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 268 | Nucleophile | |||
Binding site | 269 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 272 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 310 | L-glutamine (UniProtKB | ChEBI) | |||
Binding site | 313 | L-glutamine (UniProtKB | ChEBI) | |||
Active site | 351 | ||||
Active site | 353 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | carbamoyl-phosphate synthase complex | |
Molecular Function | ATP binding | |
Molecular Function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | arginine biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyl phosphate synthase small chain
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Campylobacteraceae > Campylobacter
Accessions
- Primary accessionA0A424Z0B6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-131 | Carbamoyl-phosphate synthase small subunit N-terminal | |||
Region | 1-187 | CPSase | |||
Sequence similarities
Belongs to the CarA family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)42,149
- Last updated2019-05-08 v1
- Checksum3E8C60BBE206BB0E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP031611 EMBL· GenBank· DDBJ | AXP08856.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WHMG01000010 EMBL· GenBank· DDBJ | MPV95799.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WHMF01000009 EMBL· GenBank· DDBJ | MPV98565.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QURW01000009 EMBL· GenBank· DDBJ | RQD87479.1 EMBL· GenBank· DDBJ | Genomic DNA |