A0A420I6H1 · A0A420I6H1_9PEZI
- ProteinSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids402 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Catalytic activity
- ATP + CoA + succinate = ADP + phosphate + succinyl-CoA
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 37 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 44-46 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GRG | ||||||
Binding site | 105 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 197 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 211 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 262 | substrate; ligand shared with subunit alpha | ||||
Sequence: N | ||||||
Binding site | 319-321 | substrate; ligand shared with subunit alpha | ||||
Sequence: GIV |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrion | |
Cellular Component | succinate-CoA ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Biological Process | succinyl-CoA metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Erysiphales > Erysiphaceae > Erysiphe
Accessions
- Primary accessionA0A420I6H1
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-200 | ATP-grasp fold succinyl-CoA synthetase-type | ||||
Sequence: FSQYGVGVPKGSVAKTGAQAEIAAKQIGGDDLVIKAQVLAGGRGKGIFDNGFKGGVRVLYSQSDAKPLAEKMIGHNLITKQTGAHGRPCNSVYICERKFARREFYLAILMDRASAAPVIVSSSQGGMDIETVAKENPEAIKTTRINIHEGVTDKIAREISKDLGFSERCIEDAMVVIKRLYQLFVEKDATQVEINPLS | ||||||
Domain | 260-380 | ATP-citrate synthase/succinyl-CoA ligase C-terminal | ||||
Sequence: VVNGAGLAMATMDIIKLNGGDPANFLDVGGNATPKAIYNAFTLITSDPKVTAIFVNIFGGIVRCDAIAEGLISIVKQMNLRVPIIVRLKGTNMEVANQLIAESGLKIFSIDDLQTAAEKSV |
Sequence similarities
Belongs to the succinate/malate CoA ligase beta subunit family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length402
- Mass (Da)43,233
- Last updated2019-05-08 v1
- Checksum9B1CDDBD06CB0E9B
Keywords
- Technical term