A0A401SMY3 · A0A401SMY3_CHIPU

Function

function

ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated. Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.
    EC:5.6.1.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site325-332ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentmembrane
Cellular Componentmicrotubule
Cellular Componentnucleus
Cellular Componentperinuclear region of cytoplasm
Cellular Componentspindle
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionisomerase activity
Molecular Functionmicrotubule binding
Molecular Functionmicrotubule severing ATPase activity
Biological Processaxonogenesis
Biological Processcytokinetic process
Biological Processendoplasmic reticulum to Golgi vesicle-mediated transport
Biological Processmicrotubule severing
Biological Processpositive regulation of microtubule depolymerization
Biological Processprotein hexamerization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Spastin
  • EC number

Gene names

    • Name
      SPAST
    • Synonyms
      SPG4
    • ORF names
      chiPu_0010189

Organism names

Accessions

  • Primary accession
    A0A401SMY3

Proteomes

Subcellular Location

Membrane
; Peripheral membrane protein
Cytoplasm, cytoskeleton
Cytoplasm, perinuclear region
Nucleus
Note: Forms an intramembrane hairpin-like structure in the membrane.

Features

Showing features for topological domain, transmembrane, intramembrane.

Type
IDPosition(s)Description
Topological domain1-39Cytoplasmic
Transmembrane33-61Helical
Intramembrane40-60Helical
Topological domain61-561Cytoplasmic

Keywords

Interaction

Subunit

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region1-23Disordered
Domain85-159MIT
Region150-256Disordered
Compositional bias178-249Polar residues
Domain317-453AAA+ ATPase

Sequence similarities

Belongs to the AAA ATPase family. Spastin subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    561
  • Mass (Da)
    62,059
  • Last updated
    2019-05-08 v1
  • Checksum
    17F92D5274049D0E
MNTPGGRGRKKKGPVRVPPDGTSDRLSLHKRNLYYFAYPLIAVFSFLRFLAFHLWLFFAYVCQRLAAMASRKAAPSITTAEPEQIRQSHKLAFDYISTALKIDEDDKGQKEKAVEWYKKGIAELEHGIAIQISGSEQMLACARFSKPEREVCRDNSGPPCPSARIQSESGAVPKKKDPLTHTSNSLPRSKYTAKITSPGQRGHQRAQSHSGISNGTGSSRMPAQPSTSTAKATSTKSTIRPNKPTTPSATPRKKMDLKNFRNVDSKLANVILNEVVDSAPSVRFEDIAGQELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVASESNATFFNISAASLTSKYVGEGEKLVRALFSAARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSSGDDRVLVMGATNRPQELDEAVLRRFTKRVFVSLPNEKTRLLLITNLLAKHGNPLSRKDLEHLAKQTEGYSGSDLTALAKDAALGPIRELKPEEVRNMAASEVRKIRLSDFEDSRRKIKRSVGPQTLEGYKRWNEEYGDTAVNS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias178-249Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BEZZ01000385
EMBL· GenBank· DDBJ
GCC31728.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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