A0A401ETL2 · EXGAL_BIFL2

Function

function

Involved in the type II arabinogalactan (AG) side chains degradation (PubMed:30564851).
Specifically releases the non-reducing terminal beta-1,6-galactobiose (beta-1,6-Gal2) from both dearabinosylated larch AG and polymeric beta-1,6-galactan chains by an exo-mode of action (PubMed:30564851).
Shows lower activity with larch AG, and very weak activity with dearabinosylated gum arabic, gum arabic and potato galactan (PubMed:30564851).
Can probably release beta-1,6-Gal2 from the internal side chains of type II AG (PubMed:30564851).

Catalytic activity

  • Hydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield (1->6)-beta-galactobiose as the final product.
    EC:3.2.1.213 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.549 mMbeta-1,6-Gal3
kcat is 261 sec-1 with beta-1,6-Gal3 as substrate.

pH Dependence

Optimum pH is 4.5 with beta-1,6-Gal3 as substrate.

Temperature Dependence

Optimum temperature is 40 degrees Celsius with beta-1,6-Gal3 as substrate.

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionhydrolase activity, hydrolyzing O-glycosyl compounds

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Exo-beta-1,6-galactobiohydrolase
  • EC number
  • Alternative names
    • Galactan exo-1,6-beta-galactobiohydrolase (non-reducing end)

Gene names

    • Name
      bl1,6Gal
    • Ordered locus names
      BLLJ_1841

Organism names

Accessions

  • Primary accession
    A0A401ETL2

Subcellular Location

Cell membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1489-1509Helical

Keywords

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-31
ChainPRO_501952696232-1513Exo-beta-1,6-galactobiohydrolase

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain666-801Ricin B-type lectin
Domain965-1112F5/8 type C 1
Domain1116-1273F5/8 type C 2
Region1456-1480Disordered

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    1,513
  • Mass (Da)
    159,668
  • Last updated
    2019-05-08 v1
  • Checksum
    C2686AF26A5093A0
MRVLSKSLAAMVAAATLVGGGAFAVAGTAYAADNDAITVTPNPWYANSFDGWGTSLAWFANATGSLGEESAITTNLGDDASKAKAVEYGKQLREQFYQSIFGDEGLDLNMARYNVGGGNASDVAYGYPFMRQGAAVPGTWKDDATGSGTYGNGVTTKQADKDKLAAAFDPTDDNQYDFSKSAAQDWWIERGATGDNPDITDVEAFANSAPWFLTNSGYATGGRNSGSNNLANPEKFAQYMAKNVEHLESLGANVDTVEPFNESETSYWGTPGDMASKYTDESDDNTKLINNYWDKYYSDKDRSVTPYSNALKKPQEGMHVSNAQQQQTITALAEALKDNDDTIIAATDATNSADFVKSYNQYPQAIKDLIGQYNVHAYSDSNQMQSRDIAQADGKKLSMSEVDGSWQSGSYNPYGFDNALGMMSKISSNVTRLQSKDFTFWQVVEDLYNMQMGSNVNPAGENTNWGTVLIDFDCTVAGMDGKLYSERRVNNNGGTTDGLEPCTVIANAKYNGVKAITHFIHAGDKVIANNDEDNNMTATSDDGKTQTVIHRNSGTSDQTFVIDLSKYGEIADNAYGELYLTTETSAEDKNAGVDSATPEVFAKTSNVKQAEGSVMIDKAAKTATVTVPARSIASIQLTGVTGYAKDAAVETGDTYQLVGKQSGKAVADTTSGDSALSLANVASDAENAKKQTWTFTQIEQPTDSERPDLKAYVITNAEGKVLVSKDGTNALSNETVGAAKSDPAAKWILNTSDGSTYQLLNAATKTNLDVDNSGTTVGTKVGLWQSPSGTSPSANQTWTLRNVTPTSQKTVNVQTAVNEKAVLPVEVTLYYTWGEGKATVANWDTSKVDVAKEGAYEATATATDVYGNEFNVTATVYVGALTVSDPVSATVLAGTSASEAKAALEAAPVYLHVKASPAFEGDAAKVTWNFDGLDTKLADAKAGDNIAVTGTYQLDDATTIALKGAIYVTAATPENVADTASNLTVTNQQTEYSKGDQWKKLTDGDTSAEAWVTWNSAGDYSASPTATIDFGSECELSSVTITYGDKAPASAKAEYTTDGETWMQFGSDVKPAAGQTVTFKADKGTVNATKMRIVNTVNNDYMNATEIQAFVTPVQGAAKNIAAASGTNFSVNFQEGASASKAIDGDTTSKGWSTWASTASTVDPVATFTFDEAQTITEVKTFFYYDGRASWPKSQTLEYQDEAGEWHGVGTKDGWKIQAGDAGSGSDGITAADTPTVDFVLGTPVKAKAIRLTNTLQDTKVYINVAEIQVFAQDSTVLTPQPASDATLGDLRLDGETVEGFDPSKTDYTVDLPVDAEANPVLQAFATDNAAAVKVTGDAVENGKLGGKAAITVTSADESETKTYTVTFNAFTLASLKVIGPTKTEYAIGDKLDTAGLKVTAVYQSGDKTKEVPVALDDPQLAIGSFDSTTAGKKAITVSYRGVTATFNVTVKANAVAPGPEEQKPGNTNKPGATGNGNKNTVANTGSSVAAIAGAVALLAAAAGALFMLRKRA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BR001494
EMBL· GenBank· DDBJ
FAA01256.1
EMBL· GenBank· DDBJ
Genomic DNA
AP010888
EMBL· GenBank· DDBJ
BAJ67505.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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