A0A3V2W3V5 · A0A3V2W3V5_SALHA
- ProteinRecBCD enzyme subunit RecB
- GenerecB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1181 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.
Miscellaneous
In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'-3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23-30 | ATP (UniProtKB | ChEBI) | |||
Binding site | 956 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 1067 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 1080 | For nuclease activity | |||
Binding site | 1080 | Mg2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | exodeoxyribonuclease V complex | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | double-stranded DNA helicase activity | |
Molecular Function | exodeoxyribonuclease V activity | |
Molecular Function | forked DNA-dependent helicase activity | |
Molecular Function | four-way junction helicase activity | |
Molecular Function | isomerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | single-stranded 3'-5' DNA helicase activity | |
Biological Process | double-strand break repair via homologous recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRecBCD enzyme subunit RecB
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3V2W3V5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-850 | DNA-binding and helicase activity, interacts with RecC | |||
Domain | 2-450 | UvrD-like helicase ATP-binding | |||
Domain | 480-746 | UvrD-like helicase C-terminal | |||
Region | 900-1181 | Nuclease activity, interacts with RecD and RecA | |||
Domain
The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.
The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.
Sequence similarities
Belongs to the helicase family. UvrD subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,181
- Mass (Da)133,843
- Last updated2019-07-31 v1
- MD5 Checksum41F071A3AC59DCFA532FAAAC9DDB969E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAGTPS010000001 EMBL· GenBank· DDBJ | EBR8515209.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHDZG010000008 EMBL· GenBank· DDBJ | EBV0400191.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHEKO010000038 EMBL· GenBank· DDBJ | EBV1618093.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFVA010000009 EMBL· GenBank· DDBJ | EBV6244276.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFVH010000007 EMBL· GenBank· DDBJ | EBV6261915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGAK010000017 EMBL· GenBank· DDBJ | EBV6844464.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGBH010000006 EMBL· GenBank· DDBJ | EBV7005795.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGIW010000001 EMBL· GenBank· DDBJ | EBV7663733.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGJI010000008 EMBL· GenBank· DDBJ | EBV7813858.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGLY010000017 EMBL· GenBank· DDBJ | EBV8260488.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGON010000007 EMBL· GenBank· DDBJ | EBV8588866.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGSF010000006 EMBL· GenBank· DDBJ | EBV9029468.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHHID010000001 EMBL· GenBank· DDBJ | EBW5810643.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHYEX010000006 EMBL· GenBank· DDBJ | ECB6296911.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAIKEG010000007 EMBL· GenBank· DDBJ | ECF1312549.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAIKRN010000001 EMBL· GenBank· DDBJ | ECF2868499.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKIVL010000021 EMBL· GenBank· DDBJ | ECS2032798.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKJSR010000002 EMBL· GenBank· DDBJ | ECS4851943.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKJUD010000016 EMBL· GenBank· DDBJ | ECS4947087.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKMBR010000003 EMBL· GenBank· DDBJ | ECT2185157.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAGAR010000006 EMBL· GenBank· DDBJ | HAB2281253.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAGSQ010000010 EMBL· GenBank· DDBJ | HAB4397590.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAGZJ010000012 EMBL· GenBank· DDBJ | HAB5201211.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAHEO010000008 EMBL· GenBank· DDBJ | HAB5788078.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQZI010000003 EMBL· GenBank· DDBJ | HAE1521974.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARDU010000016 EMBL· GenBank· DDBJ | HAE2024007.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARRO010000004 EMBL· GenBank· DDBJ | HAE3645169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASDA010000007 EMBL· GenBank· DDBJ | HAE5016385.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLP010000012 EMBL· GenBank· DDBJ | HAE6027127.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASSM010000008 EMBL· GenBank· DDBJ | HAE6816606.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASSE010000003 EMBL· GenBank· DDBJ | HAE6840211.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPD010000012 EMBL· GenBank· DDBJ | HAE8988460.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPM010000007 EMBL· GenBank· DDBJ | HAE9576640.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VLRT01000009 EMBL· GenBank· DDBJ | TSH02476.1 EMBL· GenBank· DDBJ | Genomic DNA |