A0A3V2G063 · A0A3V2G063_SALET
- ProteintRNA sulfurtransferase
- GenethiI
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids482 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
Catalytic activity
- [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]
- [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase]
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uracil-4 sulfurtransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiamine diphosphate biosynthetic process | |
Biological Process | thiazole biosynthetic process | |
Biological Process | tRNA 4-thiouridine biosynthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA sulfurtransferase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3V2G063
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 344↔456 | Redox-active | ||||
Sequence: CGVISKSPTVKAIKAKIEAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVETVSGFGPNDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWC |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 61-165 | THUMP | ||||
Sequence: LAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFSSIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGR | ||||||
Domain | 404-480 | Rhodanese | ||||
Sequence: FGPNDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFENVKVY |
Sequence similarities
Belongs to the ThiI family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length482
- Mass (Da)54,861
- Last updated2019-05-08 v1
- ChecksumC5904AE5D6B17D68
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHCWL010000006 EMBL· GenBank· DDBJ | EBU6914765.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFAS010000001 EMBL· GenBank· DDBJ | EBV3759998.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGTQ010000004 EMBL· GenBank· DDBJ | EBV9200032.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIGD010000004 EMBL· GenBank· DDBJ | EBW4104390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIXO010000007 EMBL· GenBank· DDBJ | EBW6732670.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHKXZ010000006 EMBL· GenBank· DDBJ | EBX3355174.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHOXC010000004 EMBL· GenBank· DDBJ | EBY7384468.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHPAD010000004 EMBL· GenBank· DDBJ | EBY7626553.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHWIJ010000005 EMBL· GenBank· DDBJ | ECB0523596.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLND010000003 EMBL· GenBank· DDBJ | ECT0409282.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPQX010000006 EMBL· GenBank· DDBJ | ECU3483849.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKTKA010000005 EMBL· GenBank· DDBJ | ECV4804978.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIQJ010000006 EMBL· GenBank· DDBJ | EDA0174312.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLSV010000004 EMBL· GenBank· DDBJ | EDA9401463.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLTD010000005 EMBL· GenBank· DDBJ | EDA9436675.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMGJL010000005 EMBL· GenBank· DDBJ | EDH0979263.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMIHV010000002 EMBL· GenBank· DDBJ | EDH6427905.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAMJL010000005 EMBL· GenBank· DDBJ | HAC6916530.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLP010000005 EMBL· GenBank· DDBJ | HAE2913949.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUC010000005 EMBL· GenBank· DDBJ | HAE3939200.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUZ010000008 EMBL· GenBank· DDBJ | HAE4048531.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLG010000006 EMBL· GenBank· DDBJ | HAE6001239.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLQ010000007 EMBL· GenBank· DDBJ | HAE6030545.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASRW010000007 EMBL· GenBank· DDBJ | HAE6747575.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPN010000006 EMBL· GenBank· DDBJ | HAE9562047.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAM010000027 EMBL· GenBank· DDBJ | HAF0863476.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RSGD01000007 EMBL· GenBank· DDBJ | MID45716.1 EMBL· GenBank· DDBJ | Genomic DNA |