A0A3V2G063 · A0A3V2G063_SALET

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site456Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      A4W49_07965
      , A4W57_07620
      , AIK92_12255
      , ASH82_05215
      , CB395_03590
      , CIL65_03585
      , D6J51_07995
      , D6K67_08930
      , DKU10_08575
      , DOJ23_00335
      , DP829_12645
      , DPJ44_09720
      , DPS10_09930
      , DQQ63_04285
      , DY868_14330
      , EUX26_10550
      , F9G64_05585
      , G0D76_15280
      , G3408_003097
      , G4B28_001143
      , G4B35_001643
      , G4I58_002859
      , G4I61_000645
      , G4L02_003334
      , G4Y23_002797
      , G9C15_002677
      , GCZ77_13845

Organism names

Accessions

  • Primary accession
    A0A3V2G063
  • Secondary accessions
    • A0A6C8XKB7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond344↔456Redox-active

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain404-480Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    54,861
  • Last updated
    2019-05-08 v1
  • Checksum
    C5904AE5D6B17D68
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVVRHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALAQYREQLEGKTFCVRVKRRGKHEFSSIEVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLLLIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTEDFARTMPEYCGVISKSPTVKAIKAKIEAEEENFDFSILDKVVEEANNVDIREIAQQTQQEVVEVETVSGFGPNDVILDIRSVDEQDDKPLKVEGVDVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFENVKVYRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHCWL010000006
EMBL· GenBank· DDBJ
EBU6914765.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHFAS010000001
EMBL· GenBank· DDBJ
EBV3759998.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHGTQ010000004
EMBL· GenBank· DDBJ
EBV9200032.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIGD010000004
EMBL· GenBank· DDBJ
EBW4104390.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIXO010000007
EMBL· GenBank· DDBJ
EBW6732670.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHKXZ010000006
EMBL· GenBank· DDBJ
EBX3355174.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHOXC010000004
EMBL· GenBank· DDBJ
EBY7384468.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHPAD010000004
EMBL· GenBank· DDBJ
EBY7626553.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHWIJ010000005
EMBL· GenBank· DDBJ
ECB0523596.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKLND010000003
EMBL· GenBank· DDBJ
ECT0409282.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPQX010000006
EMBL· GenBank· DDBJ
ECU3483849.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKTKA010000005
EMBL· GenBank· DDBJ
ECV4804978.1
EMBL· GenBank· DDBJ
Genomic DNA
AALIQJ010000006
EMBL· GenBank· DDBJ
EDA0174312.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLSV010000004
EMBL· GenBank· DDBJ
EDA9401463.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLTD010000005
EMBL· GenBank· DDBJ
EDA9436675.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMGJL010000005
EMBL· GenBank· DDBJ
EDH0979263.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMIHV010000002
EMBL· GenBank· DDBJ
EDH6427905.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAMJL010000005
EMBL· GenBank· DDBJ
HAC6916530.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARLP010000005
EMBL· GenBank· DDBJ
HAE2913949.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUC010000005
EMBL· GenBank· DDBJ
HAE3939200.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUZ010000008
EMBL· GenBank· DDBJ
HAE4048531.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLG010000006
EMBL· GenBank· DDBJ
HAE6001239.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLQ010000007
EMBL· GenBank· DDBJ
HAE6030545.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASRW010000007
EMBL· GenBank· DDBJ
HAE6747575.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATPN010000006
EMBL· GenBank· DDBJ
HAE9562047.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUAM010000027
EMBL· GenBank· DDBJ
HAF0863476.1
EMBL· GenBank· DDBJ
Genomic DNA
RSGD01000007
EMBL· GenBank· DDBJ
MID45716.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp