A0A3V2FZT0 · A0A3V2FZT0_SALET
- ProteinDNA gyrase subunit A
- GenegyrA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids878 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 122 | O-(5'-phospho-DNA)-tyrosine intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Cellular Component | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA negative supercoiling activity | |
Biological Process | DNA topological change | |
Biological Process | DNA-templated DNA replication |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA gyrase subunit A
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3V2FZT0
- Secondary accessions
Proteomes
Subcellular Location
Interaction
Subunit
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
Structure
Family & Domains
Features
Showing features for domain, motif, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 11-499 | DNA topoisomerase type IIA | |||
Motif | 560-566 | GyrA-box | |||
Region | 844-878 | Disordered | |||
Compositional bias | 845-878 | Acidic residues | |||
Sequence similarities
Belongs to the type II topoisomerase GyrA/ParC subunit family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length878
- Mass (Da)97,021
- Last updated2019-05-08 v1
- MD5 ChecksumAB4B575BD780AE2989C41C78FF545A26
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 845-878 | Acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHCWL010000010 EMBL· GenBank· DDBJ | EBU6915617.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFAS010000021 EMBL· GenBank· DDBJ | EBV3762142.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGTQ010000018 EMBL· GenBank· DDBJ | EBV9202025.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIGD010000007 EMBL· GenBank· DDBJ | EBW4105096.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIXO010000004 EMBL· GenBank· DDBJ | EBW6732160.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHKXZ010000010 EMBL· GenBank· DDBJ | EBX3356029.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHOXC010000010 EMBL· GenBank· DDBJ | EBY7385746.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHPAD010000010 EMBL· GenBank· DDBJ | EBY7627843.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHWIJ010000011 EMBL· GenBank· DDBJ | ECB0524877.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLND010000002 EMBL· GenBank· DDBJ | ECT0409208.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPQX010000003 EMBL· GenBank· DDBJ | ECU3483182.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIQJ010000019 EMBL· GenBank· DDBJ | EDA0175788.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLSV010000009 EMBL· GenBank· DDBJ | EDA9402525.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLTD010000008 EMBL· GenBank· DDBJ | EDA9437402.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMGJL010000004 EMBL· GenBank· DDBJ | EDH0979115.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAMJL010000009 EMBL· GenBank· DDBJ | HAC6917506.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLP010000006 EMBL· GenBank· DDBJ | HAE2914319.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUC010000002 EMBL· GenBank· DDBJ | HAE3938739.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUZ010000046 EMBL· GenBank· DDBJ | HAE4051159.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLG010000005 EMBL· GenBank· DDBJ | HAE6001092.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLQ010000018 EMBL· GenBank· DDBJ | HAE6031498.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASRW010000005 EMBL· GenBank· DDBJ | HAE6747076.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPN010000003 EMBL· GenBank· DDBJ | HAE9561221.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAM010000018 EMBL· GenBank· DDBJ | HAF0862839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RSGD01000028 EMBL· GenBank· DDBJ | MID47655.1 EMBL· GenBank· DDBJ | Genomic DNA |