A0A3V2FZT0 · A0A3V2FZT0_SALET

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site122O-(5'-phospho-DNA)-tyrosine intermediate

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular ComponentDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA negative supercoiling activity
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit A
  • EC number

Gene names

    • Name
      gyrA
    • ORF names
      A4W49_13515
      , A4W57_11350
      , AIK92_22460
      , ASH82_15640
      , D6J51_14645
      , D6K67_15615
      , DKU10_13020
      , DOJ23_11395
      , DP829_10030
      , DPJ44_13355
      , DPS10_14365
      , DQQ63_03900
      , DY868_10835
      , EUX26_17195
      , F9G64_13115
      , G0D76_20405
      , G3408_003482
      , G4B28_000668
      , G4B35_004353
      , G4I58_002706
      , G4I61_001620
      , G4L02_002817
      , G4Y23_001945
      , G9C15_002018
      , GCZ77_13075

Organism names

Accessions

  • Primary accession
    A0A3V2FZT0
  • Secondary accessions
    • A0A6C8XQJ5

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain, motif, region, compositional bias.

Type
IDPosition(s)Description
Domain11-499DNA topoisomerase type IIA
Motif560-566GyrA-box
Region844-878Disordered
Compositional bias845-878Acidic residues

Sequence similarities

Belongs to the type II topoisomerase GyrA/ParC subunit family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    878
  • Mass (Da)
    97,021
  • Last updated
    2019-05-08 v1
  • MD5 Checksum
    AB4B575BD780AE2989C41C78FF545A26
MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDNEDISIEGLMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKVYIRARAEVEADAKTGRETIIVHEIPYQVNKARLIEKIAELVKDKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINMVALHHGQPKIMNLKDIISAFVRHRREVVTRRTIFELRKARDRAHILEALAIALANIDPIIELIRRAPTPAEAKAALISLPWDLGNVAAMLERAGDDAARPEWLEPEFGVRDGQYYLTEQQAQAILDLRLQKLTGLEHEKLLDEYKELLEQIAELLHILGSADRLMEVIREEMELIRDQFGDERRTEITANSADINIEDLISQEDVVVTLSHQGYVKYQPLTDYEAQRRGGKGKSAARIKEEDFIDRLLVANTHDTILCFSSRGRLYWMKVYQLPEASRGARGRPIVNLLPLEANERITAILPVREYEEGVNVFMATASGTVKKTALTEFSRPRSAGIIAVNLNDGDELIGVDLTSGSDEVMLFSAAGKVVRFKEDAVRAMGRTATGVRGIKLAGDDKVVSLIIPRGEGAILTVTQNGYGKRTAADEYPTKSRATQGVISIKVTERNGSVVGAVQVDDCDQIMMITDAGTLVRTRVSEISVVGRNTQGVILIRTAEDENVVGLQRVAEPVDDEELDAIDGSVAEGDEDIAPEAESDDDVADDADE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias845-878Acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHCWL010000010
EMBL· GenBank· DDBJ
EBU6915617.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHFAS010000021
EMBL· GenBank· DDBJ
EBV3762142.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHGTQ010000018
EMBL· GenBank· DDBJ
EBV9202025.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIGD010000007
EMBL· GenBank· DDBJ
EBW4105096.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIXO010000004
EMBL· GenBank· DDBJ
EBW6732160.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHKXZ010000010
EMBL· GenBank· DDBJ
EBX3356029.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHOXC010000010
EMBL· GenBank· DDBJ
EBY7385746.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHPAD010000010
EMBL· GenBank· DDBJ
EBY7627843.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHWIJ010000011
EMBL· GenBank· DDBJ
ECB0524877.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKLND010000002
EMBL· GenBank· DDBJ
ECT0409208.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPQX010000003
EMBL· GenBank· DDBJ
ECU3483182.1
EMBL· GenBank· DDBJ
Genomic DNA
AALIQJ010000019
EMBL· GenBank· DDBJ
EDA0175788.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLSV010000009
EMBL· GenBank· DDBJ
EDA9402525.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLTD010000008
EMBL· GenBank· DDBJ
EDA9437402.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMGJL010000004
EMBL· GenBank· DDBJ
EDH0979115.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAMJL010000009
EMBL· GenBank· DDBJ
HAC6917506.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARLP010000006
EMBL· GenBank· DDBJ
HAE2914319.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUC010000002
EMBL· GenBank· DDBJ
HAE3938739.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUZ010000046
EMBL· GenBank· DDBJ
HAE4051159.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLG010000005
EMBL· GenBank· DDBJ
HAE6001092.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLQ010000018
EMBL· GenBank· DDBJ
HAE6031498.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASRW010000005
EMBL· GenBank· DDBJ
HAE6747076.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATPN010000003
EMBL· GenBank· DDBJ
HAE9561221.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUAM010000018
EMBL· GenBank· DDBJ
HAF0862839.1
EMBL· GenBank· DDBJ
Genomic DNA
RSGD01000028
EMBL· GenBank· DDBJ
MID47655.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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