A0A3V2FVU8 · A0A3V2FVU8_SALET

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site424Mg2+ 1 (UniProtKB | ChEBI); catalytic
Site449Interaction with DNA
Site452Interaction with DNA
Binding site498Mg2+ 1 (UniProtKB | ChEBI); catalytic
Binding site498Mg2+ 2 (UniProtKB | ChEBI)
Binding site500Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit B
  • EC number

Gene names

    • Name
      gyrB
    • ORF names
      A4W49_20275
      , A4W57_19590
      , AIK92_20555
      , ASH82_08650
      , CB395_18525
      , D6J51_20310
      , D6K67_21325
      , DKU10_19885
      , DOJ23_10200
      , DP829_21340
      , DPJ44_21180
      , DPS10_19930
      , DQQ63_18250
      , DY868_21935
      , EUX26_20880
      , F9G64_14980
      , G0D76_10405
      , G3408_004290
      , G4B28_002926
      , G4B35_003392
      , G4I58_004111
      , G4I61_001344
      , G4L02_004273
      , G4Y23_004196
      , G9C15_002322
      , GCZ77_21490

Organism names

Accessions

  • Primary accession
    A0A3V2FVU8
  • Secondary accessions
    • A0A6C8XNA6

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain418-533Toprim

Sequence similarities

Belongs to the type II topoisomerase GyrB family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    804
  • Mass (Da)
    89,896
  • Last updated
    2019-05-08 v1
  • MD5 Checksum
    E8CB83B5A5AE9653C05B180CC7AAA914
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKDIVVTIHADNSVSVTDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQRDGKIHRQIYEHGVPQAPLAVTGDTDKTGTMVRFWPSHETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLSEYLLENPSDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATLHANAHAPALSGEALEKLVSEYNATQKMIGRMERRFPKALLKELVYQPTLTEADLSDEQTVTRWVNALITELNEKEQHGSQWKFDVHTNTEQNLFEPIVRVRTHGVDTDYPLDHEFVTGAEYRRICTLGEKLRGLIEEDAFIERGERRQPVTSFEQALEWLVKESRRGLAIQRYKGLGEMNPDQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAANIDI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHCWL010000023
EMBL· GenBank· DDBJ
EBU6916934.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHFAS010000018
EMBL· GenBank· DDBJ
EBV3761915.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHGTQ010000007
EMBL· GenBank· DDBJ
EBV9200685.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIGD010000020
EMBL· GenBank· DDBJ
EBW4106594.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIXO010000019
EMBL· GenBank· DDBJ
EBW6734331.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHKXZ010000021
EMBL· GenBank· DDBJ
EBX3357093.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHOXC010000020
EMBL· GenBank· DDBJ
EBY7386840.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHPAD010000020
EMBL· GenBank· DDBJ
EBY7628934.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHWIJ010000019
EMBL· GenBank· DDBJ
ECB0525596.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKLND010000026
EMBL· GenBank· DDBJ
ECT0411948.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPQX010000018
EMBL· GenBank· DDBJ
ECU3485304.1
EMBL· GenBank· DDBJ
Genomic DNA
AALIQJ010000024
EMBL· GenBank· DDBJ
EDA0176142.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLSV010000021
EMBL· GenBank· DDBJ
EDA9403845.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLTD010000021
EMBL· GenBank· DDBJ
EDA9438988.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMGJL010000013
EMBL· GenBank· DDBJ
EDH0980724.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMIHV010000024
EMBL· GenBank· DDBJ
EDH6430803.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAMJL010000003
EMBL· GenBank· DDBJ
HAC6915602.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARLP010000012
EMBL· GenBank· DDBJ
HAE2915095.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUC010000020
EMBL· GenBank· DDBJ
HAE3940924.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUZ010000025
EMBL· GenBank· DDBJ
HAE4050229.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLG010000014
EMBL· GenBank· DDBJ
HAE6002443.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLQ010000015
EMBL· GenBank· DDBJ
HAE6031227.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASRW010000013
EMBL· GenBank· DDBJ
HAE6748478.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATPN010000014
EMBL· GenBank· DDBJ
HAE9563388.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUAM010000022
EMBL· GenBank· DDBJ
HAF0863130.1
EMBL· GenBank· DDBJ
Genomic DNA
RSGD01000020
EMBL· GenBank· DDBJ
MID47297.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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