A0A3V2FVU8 · A0A3V2FVU8_SALET
- ProteinDNA gyrase subunit B
- GenegyrB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids804 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Miscellaneous
Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )
Note: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 424 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | |||
Site | 449 | Interaction with DNA | |||
Site | 452 | Interaction with DNA | |||
Binding site | 498 | Mg2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 498 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 500 | Mg2+ 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA topological change | |
Biological Process | DNA-templated DNA replication |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA gyrase subunit B
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3V2FVU8
- Secondary accessions
Proteomes
Subcellular Location
Interaction
Subunit
Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
Structure
Sequence
- Sequence statusComplete
- Length804
- Mass (Da)89,896
- Last updated2019-05-08 v1
- MD5 ChecksumE8CB83B5A5AE9653C05B180CC7AAA914
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHCWL010000023 EMBL· GenBank· DDBJ | EBU6916934.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFAS010000018 EMBL· GenBank· DDBJ | EBV3761915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGTQ010000007 EMBL· GenBank· DDBJ | EBV9200685.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIGD010000020 EMBL· GenBank· DDBJ | EBW4106594.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIXO010000019 EMBL· GenBank· DDBJ | EBW6734331.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHKXZ010000021 EMBL· GenBank· DDBJ | EBX3357093.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHOXC010000020 EMBL· GenBank· DDBJ | EBY7386840.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHPAD010000020 EMBL· GenBank· DDBJ | EBY7628934.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHWIJ010000019 EMBL· GenBank· DDBJ | ECB0525596.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLND010000026 EMBL· GenBank· DDBJ | ECT0411948.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPQX010000018 EMBL· GenBank· DDBJ | ECU3485304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIQJ010000024 EMBL· GenBank· DDBJ | EDA0176142.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLSV010000021 EMBL· GenBank· DDBJ | EDA9403845.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLTD010000021 EMBL· GenBank· DDBJ | EDA9438988.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMGJL010000013 EMBL· GenBank· DDBJ | EDH0980724.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMIHV010000024 EMBL· GenBank· DDBJ | EDH6430803.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAMJL010000003 EMBL· GenBank· DDBJ | HAC6915602.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLP010000012 EMBL· GenBank· DDBJ | HAE2915095.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUC010000020 EMBL· GenBank· DDBJ | HAE3940924.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUZ010000025 EMBL· GenBank· DDBJ | HAE4050229.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLG010000014 EMBL· GenBank· DDBJ | HAE6002443.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLQ010000015 EMBL· GenBank· DDBJ | HAE6031227.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASRW010000013 EMBL· GenBank· DDBJ | HAE6748478.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPN010000014 EMBL· GenBank· DDBJ | HAE9563388.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAM010000022 EMBL· GenBank· DDBJ | HAF0863130.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RSGD01000020 EMBL· GenBank· DDBJ | MID47297.1 EMBL· GenBank· DDBJ | Genomic DNA |