A0A3V2FVK8 · A0A3V2FVK8_SALET
- ProteinRibonuclease E
- Generne
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1067 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per homotetramer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 303 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 346 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 404 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: C | ||||||
Binding site | 407 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic side of plasma membrane | |
Molecular Function | magnesium ion binding | |
Molecular Function | ribonuclease E activity | |
Molecular Function | RNA endonuclease activity | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | mRNA catabolic process | |
Biological Process | rRNA processing | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease E
- EC number
- Short namesRNase E
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3V2FVK8
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Subunit
Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-119 | S1 motif | ||||
Sequence: ANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTF | ||||||
Region | 404-407 | Required for zinc-mediated homotetramerization and catalytic activity | ||||
Sequence: CPRC | ||||||
Region | 585-730 | Disordered | ||||
Sequence: TKAVETAAPKAEEKAERQQDRRKPRQNNRRDRNERRDTRDNRAGRDGGESRDDNRRNRRQAQQQNAEARDTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQAQQEVKALNREEQPVQETEQEERVQQVQPRRKQRQLNQKVRF | ||||||
Compositional bias | 589-717 | Basic and acidic residues | ||||
Sequence: ETAAPKAEEKAERQQDRRKPRQNNRRDRNERRDTRDNRAGRDGGESRDDNRRNRRQAQQQNAEARDTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQAQQEVKALNREEQPVQETEQEERVQQVQP | ||||||
Region | 747-766 | Disordered | ||||
Sequence: EPRPVENVEQPVPAPRTELA | ||||||
Region | 775-820 | Disordered | ||||
Sequence: DIAPEQDDSVEPRDNTGMPRRSRRSPRHLRVSGQRRRRYRDERYPT | ||||||
Compositional bias | 780-795 | Basic and acidic residues | ||||
Sequence: QDDSVEPRDNTGMPRR | ||||||
Region | 1010-1067 | Disordered | ||||
Sequence: NHASAPMTRAPAPEYVPETPHHSDWQRPSFHFEGKGAAGGHSATRHASAPATRPQPVE |
Sequence similarities
Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,067
- Mass (Da)119,392
- Last updated2019-05-08 v1
- Checksum9914E5FA3E98339B
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 589-717 | Basic and acidic residues | ||||
Sequence: ETAAPKAEEKAERQQDRRKPRQNNRRDRNERRDTRDNRAGRDGGESRDDNRRNRRQAQQQNAEARDTRQQETAEKVKTGDEQQQTPRRERSRRRNDDKRQAQQEVKALNREEQPVQETEQEERVQQVQP | ||||||
Compositional bias | 780-795 | Basic and acidic residues | ||||
Sequence: QDDSVEPRDNTGMPRR |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHFAS010000017 EMBL· GenBank· DDBJ | EBV3761841.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIGD010000001 EMBL· GenBank· DDBJ | EBW4102877.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIXO010000001 EMBL· GenBank· DDBJ | EBW6730602.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHKXZ010000004 EMBL· GenBank· DDBJ | EBX3354614.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHOXC010000002 EMBL· GenBank· DDBJ | EBY7383641.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHPAD010000001 EMBL· GenBank· DDBJ | EBY7625204.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHWIJ010000002 EMBL· GenBank· DDBJ | ECB0522393.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLND010000001 EMBL· GenBank· DDBJ | ECT0408508.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPQX010000001 EMBL· GenBank· DDBJ | ECU3481314.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIQJ010000001 EMBL· GenBank· DDBJ | EDA0173271.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLSV010000001 EMBL· GenBank· DDBJ | EDA9399945.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLTD010000004 EMBL· GenBank· DDBJ | EDA9436276.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMGJL010000001 EMBL· GenBank· DDBJ | EDH0976922.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMIHV010000018 EMBL· GenBank· DDBJ | EDH6430287.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLP010000001 EMBL· GenBank· DDBJ | HAE2911297.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUC010000016 EMBL· GenBank· DDBJ | HAE3940566.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUZ010000005 EMBL· GenBank· DDBJ | HAE4048157.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLG010000002 EMBL· GenBank· DDBJ | HAE5999593.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLQ010000009 EMBL· GenBank· DDBJ | HAE6030761.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASRW010000001 EMBL· GenBank· DDBJ | HAE6744815.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPN010000001 EMBL· GenBank· DDBJ | HAE9559539.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAM010000021 EMBL· GenBank· DDBJ | HAF0863045.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RSGD01000001 EMBL· GenBank· DDBJ | MID43516.1 EMBL· GenBank· DDBJ | Genomic DNA |