A0A3T3GBS2 · A0A3T3GBS2_SALET

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site109Proton acceptor
Binding site129[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site133[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site136[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site183-184S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site215S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site237-239S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site316S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site359S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      trmG
    • Synonyms
      rlmN
    • ORF names
      A4W49_19175
      , A4W57_17670
      , AIK92_18695
      , ASH82_14845
      , CB395_13585
      , CIL65_17910
      , D6J51_18260
      , D6K67_19290
      , DKU10_15940
      , DOJ23_09170
      , DP829_19840
      , DPJ44_18255
      , DPS10_16525
      , DQQ63_11410
      , DY868_09465
      , EUX26_18070
      , F9G64_12360
      , G0D76_22260
      , G3408_004099
      , G4B28_002419
      , G4B35_002717
      , G4I58_003987
      , G4I61_002696
      , G4L02_002554
      , G4Y23_001682
      , G9C15_001676
      , GCZ77_11770

Organism names

Accessions

  • Primary accession
    A0A3T3GBS2
  • Secondary accessions
    • A0A6C8XLV1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond122↔359(transient)

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain115-354Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    388
  • Mass (Da)
    43,512
  • Last updated
    2019-05-08 v1
  • Checksum
    02D5566B8DAED67C
MSEQIVTPESSTPVVLNNETKINLLDLNRQQMREFFKNLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEVIDIKAI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHCWL010000015
EMBL· GenBank· DDBJ
EBU6916173.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHFAS010000015
EMBL· GenBank· DDBJ
EBV3761717.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHGTQ010000016
EMBL· GenBank· DDBJ
EBV9201869.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIGD010000014
EMBL· GenBank· DDBJ
EBW4106034.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIXO010000016
EMBL· GenBank· DDBJ
EBW6734039.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHKXZ010000014
EMBL· GenBank· DDBJ
EBX3356439.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHOXC010000016
EMBL· GenBank· DDBJ
EBY7386447.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHPAD010000016
EMBL· GenBank· DDBJ
EBY7628542.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHWIJ010000013
EMBL· GenBank· DDBJ
ECB0525048.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKLND010000012
EMBL· GenBank· DDBJ
ECT0410638.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPQX010000003
EMBL· GenBank· DDBJ
ECU3482922.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKTKA010000035
EMBL· GenBank· DDBJ
ECV4807704.1
EMBL· GenBank· DDBJ
Genomic DNA
AALIQJ010000017
EMBL· GenBank· DDBJ
EDA0175637.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLSV010000018
EMBL· GenBank· DDBJ
EDA9403637.1
EMBL· GenBank· DDBJ
Genomic DNA
AALLTD010000017
EMBL· GenBank· DDBJ
EDA9438620.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMGJL010000004
EMBL· GenBank· DDBJ
EDH0978866.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMIHV010000014
EMBL· GenBank· DDBJ
EDH6429849.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAMJL010000012
EMBL· GenBank· DDBJ
HAC6917857.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARLP010000010
EMBL· GenBank· DDBJ
HAE2914915.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUC010000015
EMBL· GenBank· DDBJ
HAE3940432.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUZ010000017
EMBL· GenBank· DDBJ
HAE4049571.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLG010000012
EMBL· GenBank· DDBJ
HAE6002328.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASLQ010000037
EMBL· GenBank· DDBJ
HAE6032542.1
EMBL· GenBank· DDBJ
Genomic DNA
DAASRW010000005
EMBL· GenBank· DDBJ
HAE6746825.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATPN010000003
EMBL· GenBank· DDBJ
HAE9560970.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUAM010000014
EMBL· GenBank· DDBJ
HAF0862510.1
EMBL· GenBank· DDBJ
Genomic DNA
RSGD01000015
EMBL· GenBank· DDBJ
MID46949.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp