A0A3T3GBS2 · A0A3T3GBS2_SALET
- ProteinDual-specificity RNA methyltransferase RlmN
- GenetrmG
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids388 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.
Miscellaneous
Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.
Catalytic activity
- adenosine2503 in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine2503 in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine
RHEA-COMP:10152 CHEBI:74411 Position: 2503+ 2 RHEA-COMP:10001 + 2 CHEBI:59789 = RHEA-COMP:10282 CHEBI:74497 Position: 2503+ CHEBI:17319 + CHEBI:57844 + 2 RHEA-COMP:10000 + CHEBI:57856
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 109 | Proton acceptor | ||||
Sequence: E | ||||||
Binding site | 129 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 133 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 136 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 183-184 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: GE | ||||||
Binding site | 215 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 237-239 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: SLH | ||||||
Binding site | 316 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 359 | S-methylcysteine intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | rRNA (adenine(2503)-C2-)-methyltransferase activity | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA (adenine(37)-C2)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | rRNA base methylation | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDual-specificity RNA methyltransferase RlmN
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3T3GBS2
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 122↔359 | (transient) | ||||
Sequence: CVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAAC |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 115-354 | Radical SAM core | ||||
Sequence: EDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGEPLLNLTNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDTIRDEIVPINKKYNIETFLGAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKETPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDD |
Sequence similarities
Belongs to the radical SAM superfamily. RlmN family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length388
- Mass (Da)43,512
- Last updated2019-05-08 v1
- Checksum02D5566B8DAED67C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAHCWL010000015 EMBL· GenBank· DDBJ | EBU6916173.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHFAS010000015 EMBL· GenBank· DDBJ | EBV3761717.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHGTQ010000016 EMBL· GenBank· DDBJ | EBV9201869.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIGD010000014 EMBL· GenBank· DDBJ | EBW4106034.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIXO010000016 EMBL· GenBank· DDBJ | EBW6734039.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHKXZ010000014 EMBL· GenBank· DDBJ | EBX3356439.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHOXC010000016 EMBL· GenBank· DDBJ | EBY7386447.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHPAD010000016 EMBL· GenBank· DDBJ | EBY7628542.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHWIJ010000013 EMBL· GenBank· DDBJ | ECB0525048.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLND010000012 EMBL· GenBank· DDBJ | ECT0410638.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPQX010000003 EMBL· GenBank· DDBJ | ECU3482922.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKTKA010000035 EMBL· GenBank· DDBJ | ECV4807704.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIQJ010000017 EMBL· GenBank· DDBJ | EDA0175637.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLSV010000018 EMBL· GenBank· DDBJ | EDA9403637.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALLTD010000017 EMBL· GenBank· DDBJ | EDA9438620.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMGJL010000004 EMBL· GenBank· DDBJ | EDH0978866.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMIHV010000014 EMBL· GenBank· DDBJ | EDH6429849.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAMJL010000012 EMBL· GenBank· DDBJ | HAC6917857.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLP010000010 EMBL· GenBank· DDBJ | HAE2914915.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUC010000015 EMBL· GenBank· DDBJ | HAE3940432.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUZ010000017 EMBL· GenBank· DDBJ | HAE4049571.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLG010000012 EMBL· GenBank· DDBJ | HAE6002328.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASLQ010000037 EMBL· GenBank· DDBJ | HAE6032542.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAASRW010000005 EMBL· GenBank· DDBJ | HAE6746825.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATPN010000003 EMBL· GenBank· DDBJ | HAE9560970.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAM010000014 EMBL· GenBank· DDBJ | HAF0862510.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RSGD01000015 EMBL· GenBank· DDBJ | MID46949.1 EMBL· GenBank· DDBJ | Genomic DNA |