A0A3S1AP81 · A0A3S1AP81_9CYAN
- ProteinReplicative DNA helicase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1121 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The intein is an endonuclease.
The main replicative DNA helicase, it participates in initiation and elongation during chromosome replication. Travels ahead of the DNA replisome, separating dsDNA into templates for DNA synthesis. A processive ATP-dependent 5'-3' DNA helicase it has DNA-dependent ATPase activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | primosome complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | endonuclease activity | |
Molecular Function | isomerase activity | |
Biological Process | DNA replication, synthesis of primer | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | intein-mediated protein splicing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicative DNA helicase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Nostocales > Calotrichaceae > Dulcicalothrix
Accessions
- Primary accessionA0A3S1AP81
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 334-554 | DOD-type homing endonuclease | |||
Domain | 416-461 | HTH cro/C1-type | |||
Domain | 719-885 | SF4 helicase | |||
Domain | 1060-1120 | SF4 helicase | |||
Sequence similarities
Belongs to the helicase family. DnaB subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,121
- Mass (Da)125,291
- Last updated2019-04-10 v1
- MD5 Checksum23A2255232D47515AB9F543AB5D45A94
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
RSCL01000004 EMBL· GenBank· DDBJ | RUT07670.1 EMBL· GenBank· DDBJ | Genomic DNA |