A0A3S0P423 · A0A3S0P423_9BACI

  • Protein
    2-isopropylmalate synthase
  • Gene
    leuA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site13Mn2+ (UniProtKB | ChEBI)
Binding site201Mn2+ (UniProtKB | ChEBI)
Binding site203Mn2+ (UniProtKB | ChEBI)
Binding site237Mn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2-isopropylmalate synthase activity
Molecular Functionacetyl-CoA C-acetyltransferase activity
Molecular Functionmanganese ion binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-isopropylmalate synthase
  • EC number
  • Alternative names
    • Alpha-IPM synthase
    • Alpha-isopropylmalate synthase

Gene names

    • Name
      leuA
    • ORF names
      EK386_10020

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SYSU K30002
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Lysinibacillus

Accessions

  • Primary accession
    A0A3S0P423

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain4-266Pyruvate carboxyltransferase
Region392-514Regulatory domain

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    514
  • Mass (Da)
    56,120
  • Last updated
    2019-04-10 v1
  • Checksum
    ACC98ED038BEE90D
MRKIDIFDTTLRDGEQSAGINLNTAEKIEIAKQLERLGVTIIEAGFPASSPGDFEAVNRIAGTVKNSIVTGLARCVKNDIDTTWEALKVAEQPHIHVFLATSPIHMEYKLKKSPDQVVEQAVEAVKYAKKFFPLVQWSAEDGFRSDRDFLVRIIGEVIAAGATTINVPDTVGYASPQEYGELFAFLRENVRGADQVKFSAHCHDDLGMAVANSIAAIQNGADQVECTINGIGERAGNAALEEIGVAMHIRNDLYQVETGLNLKEIKRTSQLVSRLTGVVIQPNKAVVGKNAFAHESGIHQDGVLKNKETYEIISPALIGEEDVPLVLGKHSGRAAFRDRAITMGFDLSDDKLNKAFQEFKKLADRKKEITEEDLMTLLTEQQVSIEDIPLFELKSVQVSYGTENIPTATASVLTPEGELKTLAATGSGSVEAIFNTLEQLVNAKVNILDFRVKSVGKGRDALGEAVINLRYNGFTSTGRDAAQDVLEATAKAYLNAINRHLIQESIRAKQTTEV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
RYYR01000011
EMBL· GenBank· DDBJ
RUL52178.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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