A0A3S0P423 · A0A3S0P423_9BACI
- Protein2-isopropylmalate synthase
- GeneleuA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids514 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | acetyl-CoA C-acetyltransferase activity | |
Molecular Function | manganese ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Lysinibacillus
Accessions
- Primary accessionA0A3S0P423
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-266 | Pyruvate carboxyltransferase | ||||
Sequence: IDIFDTTLRDGEQSAGINLNTAEKIEIAKQLERLGVTIIEAGFPASSPGDFEAVNRIAGTVKNSIVTGLARCVKNDIDTTWEALKVAEQPHIHVFLATSPIHMEYKLKKSPDQVVEQAVEAVKYAKKFFPLVQWSAEDGFRSDRDFLVRIIGEVIAAGATTINVPDTVGYASPQEYGELFAFLRENVRGADQVKFSAHCHDDLGMAVANSIAAIQNGADQVECTINGIGERAGNAALEEIGVAMHIRNDLYQVETGLNLKEIK | ||||||
Region | 392-514 | Regulatory domain | ||||
Sequence: ELKSVQVSYGTENIPTATASVLTPEGELKTLAATGSGSVEAIFNTLEQLVNAKVNILDFRVKSVGKGRDALGEAVINLRYNGFTSTGRDAAQDVLEATAKAYLNAINRHLIQESIRAKQTTEV |
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length514
- Mass (Da)56,120
- Last updated2019-04-10 v1
- ChecksumACC98ED038BEE90D