A0A3Q9FKI8 · A0A3Q9FKI8_9BACT

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site48L-glutamine (UniProtKB | ChEBI)
Binding site226L-glutamine (UniProtKB | ChEBI)
Binding site228L-glutamine (UniProtKB | ChEBI)
Active site254Nucleophile
Binding site255L-glutamine (UniProtKB | ChEBI)
Binding site258L-glutamine (UniProtKB | ChEBI)
Binding site296L-glutamine (UniProtKB | ChEBI)
Binding site298L-glutamine (UniProtKB | ChEBI)
Binding site299L-glutamine (UniProtKB | ChEBI)
Active site340
Active site342

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      EI427_00215

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • L12M1
  • Taxonomic lineage
    Bacteria > Bacteroidota > Cytophagia > Cytophagales > Flammeovirgaceae > Flammeovirga

Accessions

  • Primary accession
    A0A3Q9FKI8

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-173CPSase
Domain4-134Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    363
  • Mass (Da)
    39,990
  • Last updated
    2019-04-10 v1
  • Checksum
    A53C412DAC04C6C4
MEKKTAILMLQDGTTYKGTAIGAIGTTTGEICFNTSMTGYQEIYTDPSYYGQIIVNTVSHIGNYGTVHNENESAKPQINGLVVKSFSSVYSRNTAEGSLQDYLENANLVGIADIDTRELVRHIRQKGAMNAIITSEISDLDEIKKKLKATPDMEGLELSSVVCTKEPYSVGDPHAAVKVAVLDLGIKKSILDNFTSRGMLCKVFPSNTTFEQMKQWKPDGYFLSNGPGDPAVMDYAVETAKQILEADKPLFGICLGHQVISRAVGLSTYKMHHGHRGANHPVKNLISGRSEITSQNHGFAVDMDSIKKNDNIELTHINLNDDTVEGIKVLDKKAFSIQYHPEASPGPHDSRYLFTRFLELIQD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP034562
EMBL· GenBank· DDBJ
AZQ60684.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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