A0A3Q1NEG5 · A0A3Q1NEG5_BOVIN
- ProteinPhosphatidylethanolamine N-methyltransferase
- GenePEMT
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids227 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.
Catalytic activity
- 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + H+ + S-adenosyl-L-homocysteine
Pathway
Lipid metabolism.
Phospholipid metabolism; phosphatidylcholine biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | mitochondrial membrane | |
Molecular Function | phosphatidyl-N-dimethylethanolamine N-methyltransferase activity | |
Molecular Function | phosphatidyl-N-methylethanolamine N-methyltransferase activity | |
Molecular Function | phosphatidylethanolamine N-methyltransferase activity | |
Biological Process | methylation | |
Biological Process | phosphatidylcholine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphatidylethanolamine N-methyltransferase
- EC number
- Short namesPEAMT ; PEMT
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionA0A3Q1NEG5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Mitochondrion membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Note: Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
Features
Showing features for topological domain, intramembrane, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-40 | Lumenal | ||||
Sequence: MRLHCSNVCRTWTHQSLPACSWRADLCIMTRLLGYVDLSE | ||||||
Intramembrane | 41-61 | Helical | ||||
Sequence: PHFVAAVLAIVFNPLFWNVVA | ||||||
Transmembrane | 42-60 | Helical | ||||
Sequence: HFVAAVLAIVFNPLFWNVV | ||||||
Topological domain | 62-73 | Lumenal | ||||
Sequence: RWEHKTRKLSKA | ||||||
Transmembrane | 81-104 | Helical | ||||
Sequence: CYTLGGAILLLNVLRSHCFTQAML | ||||||
Topological domain | 95-121 | Cytoplasmic | ||||
Sequence: RSHCFTQAMLSQPRMQSLDNPAVYHVG | ||||||
Transmembrane | 116-146 | Helical | ||||
Sequence: AVYHVGLALLGVGGVFVLSSFLALGFTGTFL | ||||||
Topological domain | 143-185 | Lumenal | ||||
Sequence: GTFLGDYFGILKEARVTMFPFSVLDNPMYWGSTAIYLGWAIVH | ||||||
Topological domain | 207-227 | Cytoplasmic | ||||
Sequence: YEEPFTAEIYQQKASQAYKRS |
Keywords
- Cellular component
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length227
- Mass (Da)25,289
- Last updated2024-05-29 v2
- Checksum415017F739287DA9
Computationally mapped potential isoform sequences
There are 8 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AAA9SUE4 | A0AAA9SUE4_BOVIN | PEMT | 289 | ||
A0AAF6ZEE4 | A0AAF6ZEE4_BOVIN | PEMT | 234 | ||
A0AAA9STE9 | A0AAA9STE9_BOVIN | PEMT | 192 | ||
A0A3Q1N737 | A0A3Q1N737_BOVIN | PEMT | 264 | ||
A0AAA9SY51 | A0AAA9SY51_BOVIN | PEMT | 198 | ||
A0A3Q1MIX3 | A0A3Q1MIX3_BOVIN | PEMT | 208 | ||
A0AAA9TTX4 | A0AAA9TTX4_BOVIN | PEMT | 221 | ||
A0A3Q1M544 | A0A3Q1M544_BOVIN | PEMT | 257 |
Keywords
- Technical term