A0A3Q1MCI1 · A0A3Q1MCI1_BOVIN

  • Protein
    Cytochrome P450 2E1
  • Gene
    CYP2C87
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.

Catalytic activity

  • (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z)-eicosatrienoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + reduced [NADPH--hemoprotein reductase] + O2 = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • tetradecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 13-hydroxytetradecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
    EC:1.14.14.1 (UniProtKB | ENZYME | Rhea)
  • an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.
  • dodecanoate + reduced [NADPH--hemoprotein reductase] + O2 = 11-hydroxydodecanoate + oxidized [NADPH--hemoprotein reductase] + H2O + H+
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Activity regulation

The omega-1 hydroxylase activity is stimulated by cytochrome b5.

Pathway

Lipid metabolism; fatty acid metabolism.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site412Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentmitochondrial inner membrane
Molecular Functionaromatase activity
Molecular Functionheme binding
Molecular FunctionHsp70 protein binding
Molecular FunctionHsp90 protein binding
Molecular Functioniron ion binding
Biological Processfatty acid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      CYP2C87

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Hereford
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    A0A3Q1MCI1

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Peripheral membrane protein
Microsome membrane
; Peripheral membrane protein
Mitochondrion inner membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Features

Showing features for signal, chain.

Type
IDPosition(s)Description
Signal1-25
ChainPRO_501875714926-467Cytochrome P450 2E1

Expression

Gene expression databases

Interaction

Subunit

Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    467
  • Mass (Da)
    53,029
  • Last updated
    2019-04-10 v1
  • Checksum
    98D41816433B19CA
MDLAVVLVLCLSCLLLLSLWKQSSGKGKLPPGPTPLPILGNIFQLDVKNISKSLTSLSKVYGPVFTVYFGMKPTVVLHGYEAVKEALIDLGEEFSRRGSFPVIERNVKGHGRNFRSPPETRQEARCLVEELRKTNGLPCDPTFILGCAPCNVICSIIFQNRFDYKDQTFLNLMKTINENIKILGSPWIQVLNIFPVLLDFFPWSYSYKKLYTNTAYVKNYVLEKTREHQASLDINNPRDFIDCFLIKMEQEKHNHQSEYTFENLTITVSDLFGAGTETTSTTLRYGLLLLLKHPEVTAKIQEEIDRVIGRHRSPCMQDRTHMPYMDAVLHEIQRYIDLAPMSVPHAVNCDVKFRNYLIPKGTDILTSLTSVLHDDKEFPNPEVFDPGHFLDENGNFRKSDYFMAFSAGKRVCVGEGLARMELFLFLTTILQTFTLKSVVDPKDLDTTPAVTGIANVPPPYQLCFIPV

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A3S5ZP79A0A3S5ZP79_BOVINCYP2C87492
A0A3Q1LL00A0A3Q1LL00_BOVINCYP2C87480
A0AAA9TEA3A0AAA9TEA3_BOVINCYP2C87469
A0A3Q1MQI2A0A3Q1MQI2_BOVINCYP2C87443
A0A3Q1M6Q6A0A3Q1M6Q6_BOVINCYP2C87462

Keywords

Genome annotation databases

Similar Proteins

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