A0A3Q1M2J4 · A0A3Q1M2J4_BOVIN
- ProteinAdenylosuccinate synthetase
- GeneADSS1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids421 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Plays an important role in the de novo pathway of purine nucleotide biosynthesis.
Catalytic activity
- IMP + L-aspartate + GTP = N6-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 42-48 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GDEGKGK | ||||||
Active site | 43 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 43 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 43-46 | IMP (UniProtKB | ChEBI) | ||||
Sequence: DEGK | ||||||
Binding site | 68-71 | IMP (UniProtKB | ChEBI) | ||||
Sequence: NAGH | ||||||
Binding site | 70 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 70-72 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GHT | ||||||
Active site | 71 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 220 | IMP (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 235 | IMP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 295-301 | substrate | ||||
Sequence: VTTGRKR | ||||||
Binding site | 299 | IMP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 301 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 327-329 | GTP (UniProtKB | ChEBI) | ||||
Sequence: KLD | ||||||
Binding site | 409-411 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GVG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenylosuccinate synthase activity | |
Molecular Function | GTP binding | |
Molecular Function | magnesium ion binding | |
Biological Process | 'de novo' AMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenylosuccinate synthetase
- EC number
- Short namesAMPSase ; AdSS
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionA0A3Q1M2J4
Proteomes
Organism-specific databases
Subcellular Location
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: MSGTRASNDRPPSAGGVKRGRLQHE |
Sequence similarities
Belongs to the adenylosuccinate synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length421
- Mass (Da)46,276
- Last updated2019-04-10 v1
- Checksum238A747CD04C972F
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AAF6ZFA7 | A0AAF6ZFA7_BOVIN | ADSS1 | 472 | ||
A0AAA9SPF0 | A0AAA9SPF0_BOVIN | ADSS1 | 453 | ||
A0AAA9RUG8 | A0AAA9RUG8_BOVIN | ADSS1 | 444 |
Keywords
- Technical term