A0A3P5DM28 · A0A3P5DM28_ECOLX
- ProteinAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- GenebioA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids429 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
Catalytic activity
- (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = S-adenosyl-4-methylsulfanyl-2-oxobutanoate + (7R,8S)-7,8-diammoniononanoate
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 17 | Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM | ||||
Sequence: Y | ||||||
Binding site | 52 | substrate | ||||
Sequence: W | ||||||
Binding site | 112-113 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: GS | ||||||
Binding site | 144 | substrate | ||||
Sequence: Y | ||||||
Binding site | 245 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 274 | substrate | ||||
Sequence: K | ||||||
Binding site | 307 | substrate | ||||
Sequence: G | ||||||
Binding site | 308-309 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: PT | ||||||
Binding site | 391 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenosylmethionine-8-amino-7-oxononanoate transaminase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A3P5DM28
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 274 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length429
- Mass (Da)47,325
- Last updated2019-02-13 v1
- ChecksumB99DFA5FC314B38A