A0A3L8SRH5 · A0A3L8SRH5_CHLGU

Function

function

Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = sn-glycerol 3-phosphocholine + 2 hexadecanoate + 2 H+
    This reaction proceeds in the forward direction.
  • 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1,3-dihexadecanoylglycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-glycerol + hexadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-octadecanoyl-sn-glycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + octadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + hexadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H+
    This reaction proceeds in the forward direction.
  • 1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = 1-octadecanoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H+
    This reaction proceeds in the forward direction.
  • 2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • 2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H+
    This reaction proceeds in the forward direction.
  • a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+
    This reaction proceeds in the forward direction.
    EC:3.1.1.4 (UniProtKB | ENZYME | Rhea)
  • a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H+
    This reaction proceeds in the forward direction.
    EC:3.1.1.5 (UniProtKB | ENZYME | Rhea)
  • a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H+
    This reaction proceeds in the forward direction.
    EC:3.1.1.3 (UniProtKB | ENZYME | Rhea)

GO annotations

AspectTerm
Cellular Componentapical plasma membrane
Cellular Componentbrush border membrane
Molecular Functionlysophospholipase activity
Molecular Functionphospholipase A2 activity
Molecular Functionretinyl-palmitate esterase activity
Molecular Functiontriacylglycerol lipase activity
Biological Processphospholipid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phospholipase B1, membrane-associated
  • EC number
  • Alternative names
    • Lysophospholipase
    • Phospholipase A2
    • Phospholipase B/lipase
    • Triacylglycerol lipase

Gene names

    • ORF names
      DV515_00003982

Organism names

  • Taxonomic identifier
  • Strain
    • Red01
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Australaves > Passeriformes > Passeroidea > Passeridae > Chloebia

Accessions

  • Primary accession
    A0A3L8SRH5

Proteomes

Subcellular Location

Apical cell membrane
; Single-pass type I membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1126-1148Helical

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias25-43Polar residues
Region25-48Disordered
Compositional bias392-414Polar residues
Region392-415Disordered

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    1,170
  • Mass (Da)
    130,267
  • Last updated
    2019-02-13 v1
  • Checksum
    8FD68DE658E03B00
NGKHTLSALQENPYLFTYRNSNYSSSLRTSNRKETSQERSYGTNIPCPDRFPSDTVPVSVHSLKPADIQVIAALGDSLTAANGAGSRPHDVLDVLTQYRGLSWSIGGNENISTVTTLPNILREFNPSLVGYSIGTGTQNSNNAALNQAVTGARAEDVPKQVRKLVDRMKNDTRIDFQNDWKLITLFIGGNDLCQVCENPVHYSPENYTYNIQIALDLLHKEVPRAYVNLVTMLYIARLRELHQSENNSCPKLLMRTLCPCVINPKNDSSELKKLIYFNKMYQEGTRQLVESGRYDTKDDFTVVMQPFMTNMEMPKTQEGWPDDSYFAPDCFHFSQKTHSQAARALWNNMLEPVGEKTDSQNIDDEFVLKCPSEAEPFLRTYKNSNYTYPNQSPNYGSQLPCEDRSPSSPPATSVHSLKPADVKIIAALGDSLTAGSGIASDTLQDVITQYRGLSWSIGGDESLENVTTLPNIFREFNVTIMGYSTGTGSENDSNAFLNQAVPGALAEHLPAQARRLVSLMKTDQRIDFSADWKLITVHIGANDLCIYCKNPDHYSAGNYTKRIQETLDILHKEASTVPKALVSLVDVEDITALRHLFVDPSVQCPTYLADYLCSCVLLGEENSENLTMVRDAIKAYQLGIQRLIESGRYDTHENFTVVIQPFLQNLKIPLDQDEKPDVSYFAPDCFHPSQKGHSQLARALWNTVLQPVGQKADSFDFSADIVLGCPAQNSPFLGTYRNSNYTPVEPTREPIENWGSELSCPGHTPSSHVPTSVHELRPADIKAIGALGDSLTTAVGAKVPDLQTDWKGLSWSIGGDDTLEIQATLPNILKKFNPNLFGFSTGTSKETAGFNVAERNATARSGDPESDVLLEHTSSVLSPSDMPAQARALVELMRSSSKINFKEDWKLITILVGGSDLCQYCLDKETYSVQKYVKHLQDTLDIFYEELPRVFINMVEMLELSGLHQITASSSECALAAKKVCPCFLNPEENSSELQEIKRVNRDFQAGALGLISSRRYEQREDFAVVIQPFLRNTLLPLDTTSKPDMSFFAADCFHFSVRGYAEMAMALWNNMLEPVGEKQTYNNFTHDRSKLRCPNPEKPFLSTRRNSGFGNSDLSLEKTESSVPYWAVIVTAVAGILVGSLLGWVVSRRTTKKHQHKADTENNIKTTSL

Features

Showing features for non-terminal residue, compositional bias.

TypeIDPosition(s)Description
Non-terminal residue1
Compositional bias25-43Polar residues
Compositional bias392-414Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QUSF01000008
EMBL· GenBank· DDBJ
RLW07140.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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