A0A3L7Y2Y4 · A0A3L7Y2Y4_9CHLR
- ProteinMultifunctional fusion protein
- GenersmA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids647 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
Catalytic activity
- 4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+
Pathway
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 35 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 37 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 62 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 83 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 111 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 132 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 340 | |||||
Sequence: K | ||||||
Binding site | 423-433 | ATP (UniProtKB | ChEBI) | ||||
Sequence: PVAAGLGGGSS | ||||||
Active site | 465 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity | |
Molecular Function | 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity | |
Molecular Function | ATP binding | |
Molecular Function | RNA binding | |
Biological Process | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameRibosomal RNA small subunit methyltransferase A
- EC number
- Alternative names
- Recommended name4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
- EC number
- Short namesCMK
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Chloroflexota > Candidatus Limnocylindria > Candidatus Aquidulcis
Accessions
- Primary accessionA0A3L7Y2Y4
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 42-215 | Ribosomal RNA adenine methylase transferase N-terminal | ||||
Sequence: VRDRIVQAGAPTVGELVLEIGPGLGFLTEALLVSGAKVVAVELDSDLIPRLEHKFASQISAGQLTLIHGDALTVDLESLAPTSGSWRVIANIPYHVTSPLLHRLLLLAVPPARIVLLVQLEVAERVAAAPGDWSYLTAFVQARTAASVVARVPREAFDPVPGVDSAILLLEHRT | ||||||
Region | 306-329 | Disordered | ||||
Sequence: VARRSTSGGSDVRERNTTRSHPAA |
Sequence similarities
Belongs to the GHMP kinase family. IspE subfamily.
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length647
- Mass (Da)68,216
- Last updated2019-02-13 v1
- Checksum11434CE276A43619