A0A3G2Y4I4 · A0A3G2Y4I4_GETV

Function

function

Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Catalytic activity

  • 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = ADP-D-ribose + H+ + L-aspartyl-[protein]
    This reaction proceeds in the forward direction.
  • 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = ADP-D-ribose + H+ + L-glutamyl-[protein]
    This reaction proceeds in the forward direction.
  • ADP-alpha-D-ribose 1''-phosphate + H2O = ADP-D-ribose + phosphate
    This reaction proceeds in the forward direction.
    EC:3.1.3.84 (UniProtKB | ENZYME | Rhea)
  • ATP + H2O = ADP + H+ + phosphate
    EC:3.6.4.13 (UniProtKB | ENZYME | Rhea)
  • ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide
    EC:2.7.7.19 (UniProtKB | ENZYME | Rhea)
  • GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
  • L-histidyl-[protein] + N7-methyl-GTP = diphosphate + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein]
    This reaction proceeds in the forward direction.
  • a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ + N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
  • a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + H+ + phosphate
    This reaction proceeds in the forward direction.
    EC:3.6.1.74 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H+ + phosphate
    EC:3.6.1.15 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site1012For cysteine protease nsP2 activity
Active site1081For cysteine protease nsP2 activity

GO annotations

AspectTerm
Cellular Componentcytoplasmic vesicle membrane
Cellular Componenthost cell cytoplasmic vesicle membrane
Cellular Componenthost cell filopodium
Cellular Componenthost cell nucleus
Cellular Componenthost cell plasma membrane
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functioncysteine-type peptidase activity
Molecular FunctionGTP binding
Molecular Functionmetal ion binding
Molecular FunctionmRNA methyltransferase activity
Molecular Functionpoly(A) RNA polymerase activity
Molecular Functionpolynucleotide 5'-phosphatase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Process7-methylguanosine mRNA capping
Biological ProcessDNA-templated transcription
Biological Processmethylation
Biological ProcessmRNA modification
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
Biological Processviral RNA genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Polyprotein P1234
  • Alternative names
    • Non-structural polyprotein

Organism names

Accessions

  • Primary accession
    A0A3G2Y4I4

Subcellular Location

Cell membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Lipid-anchor
Cytoplasmic vesicle membrane
; Peripheral membrane protein
Host cell membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Lipid-anchor
Host cytoplasmic vesicle membrane
; Peripheral membrane protein
Host nucleus
Membrane
; Lipid-anchor
Membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain27-258Alphavirus-like MT
Domain689-990+RNA virus helicase C-terminal
Domain1003-1325Peptidase C9
Domain1323-1492Macro
Region1767-1804Disordered
Compositional bias1787-1801Pro residues
Domain2221-2336RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,467
  • Mass (Da)
    275,320
  • Last updated
    2019-02-13 v1
  • Checksum
    F391A483B4C01911
MKVTVDVEADSPFLKALQKAFPAFEVESQQVTPNDHANARAFSHLATKLIEQEVPTGVTILDVGSAPARRLMSDHTYHCICPMKSAEDPERLANYARKLAKASGTVLDKNVSGKITDLQDVMATPDLESPTFCLHTDETCRTRAEVAVYQDVYAVHAPTSLYHQAIKGVRTAYWIGFDTTPFMFEALAGAYPAYSTNWADEQVLQARNIGLCATGLSEGRRGKLSIMRKKCLRPSDRVMFSVGSTLYTESRKLLRSWHLPSVFHLKGKNSFTCRCDTVVSCEGYVVKKITISPGIYGKTVDYAVTHHAEGFLVCKITDTVRGERVSFPVCTYVPATICDQMTGILATDVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFSKWAREARADMEDEKPLGTRERTLTCCCLWAFKSHKTHTMYKRPETQTIVKVPSTFDSFVIPSLWSSSLSMGIRRRIKLLLSARMAQGLPYSGDRTEARAAEEEEKEVQEAELTRAALPPLVSGSCADDIAQVDVEELTFRAGAGVVETPRNALKVTPQAHDHLIGSYLILSPQTVLKSEKLAPIHPLAEQVTVMTHSGRSGRYPVDKYDGRVLIPTGAAIPVSEFQALSESATMVYNEREFINRKLHHIALYGPALNTDEESYEKVRAERAETEYVFDVDKKACIKKEEASGLVLTGDLINPPFHEFAYEGLKIRPAAPYHTTIIGVFGVPGSGKSAIIKNMVTTRDLVASGKKENCQEIMNDVKRQRGLDVTARTVDSILLNGCKKGVENLYVDEAFACHSGTLLALIALVRPSGKVVLCGDPKQCGFFNLMQLKVHYNHNICTRVLHKSISRRCTLPVTAIVSTLHYQGKMRTTNRCNTPIQIDTTGSSKPASGDIVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQKVNENPLYSPLSEHVNVLLTRTENRLVWKTLSGDPWIKVLTNVPRGDFSATLEEWQEEHDGIMRVLNERPAEVDPFQNKAKVCWAKCLVQVLETAGIRMTADEWNTILAFREDRAYSPEVALNEICTRYYGVDLDSGLFSAQSVSLSYENNHWDNRPGGRMYGFNHEVARKYAARFPFLRGNMNSGLQLNVPERKLQPFSAECNIVPSNRRLPHALVTSYQQCRGERVEWLLKKIPGHQMLLVSEYNLVIPHKRVFWIAPPRVSGADRTYDLDLGLPMDAGRYDLVFVNIHTEYRQHHYQQCVDHSMRLQMLGGDSLHLLRPGGSLLMRAYGYADRVSEMVVTALARKFSAFRVLRPACVTSNTEVFLLFSNFDNGRRAVTLHQANQKLSSMYACNGLHTAGCAPSYRVRRADISGHSEEAVVNAANAKGTVSDGVCRAVAKKWPSSFKGAATPVGTAKMIRADGMTVIHAVGPNFSTVTEAEGDRELAAAYRAVASIISTNNIKSVAVPLLSTGTFSGGKDRVMQSLNHLFTALDATDADVVIYCRDKNWEKKIQEAIDRRTAIELVSEDVTLETDLVRVHPDSCLVGRNGYSATDGKLYSYLEGTRFHQTAVDMAEISTLWPRLQDANEQICLYALGETMDSIRTKCPVEDADSSTPPKTVPCLCRYAMTAERVARLRMNNTKNIIVCSSFPLPKYRIEGVQKVKCDRVLIFDQTVPSLVSPRKYIQQPPEQLDNVSLTSTTSTGSAWSFPSETTYETMEVVAEVHTEPPIPPPRRRRAAVAQLRRDLEVTEEIEPYVTQQAEVMVMERVTTTDIRAIPVPARRAITMPVPAPRVRKVATEPPLEPEAPIPAPRKRRTTSTPPPHNPEDFVPRVPVELPWEPEDLDIQFGDLEPRRRNTRDRDVSTGIQFGDIDFNQSXLGRAGAYIFSSDTGPGHLQQKSVRQHELPCETLYAHEDERIYPPAFDGEKEKVLQAKMQMAPTEANKSRYQSRKVENMKALIVERLREGAKLYLHEQTDKVPTYTSKYPRPVYSPSVDDSLSDPEVAVAACNSFLEENYPTVANYQITDEYDAYLDLVDGSESCLDRATFCPAKLRCYPKHHAYHQPQIRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTMDSAVFNVESFKKYACTGEYWQEFKDNPIRITTENITTYVAKLKGPKAAALFAKTHNLVPLQEVPMDRFVMDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLKAVLTPNIHTLFDMSAEDFDAIIAAHFQPGDAVLETDIASFDKSQDDSLALTALMLLEDLGVDQELLDLIEAAFGEITSVHLPTGTRFKFGAMMKSGMFLTLFINTLLNIVIACRVLRDKLSSSACAAFIGDDNIVHGVRSDPLMAERCASWVNMEVKIIDATMCEKPPYFCGGFILYDSVTGTACRVADPLKRLFKLGKPLPADDNQDEDRRRALKDETVKWSRIGLREELDVALSSRYQVSGVGNITRAMSTLSKSLKSFRKIRGPIIHLYGGPK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1787-1801Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MG865968
EMBL· GenBank· DDBJ
AYP19288.1
EMBL· GenBank· DDBJ
Genomic RNA
MG865969
EMBL· GenBank· DDBJ
AYP19290.1
EMBL· GenBank· DDBJ
Genomic RNA

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