A0A3D1Z6R9 · A0A3D1Z6R9_VIBSX

  • Protein
    Ribonuclease E
  • Gene
    rne
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Organism
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site302Mg2+ (UniProtKB | ChEBI); catalytic
Binding site345Mg2+ (UniProtKB | ChEBI); catalytic
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      DEV85_10045

Organism names

  • Taxonomic identifier
  • Organism
    Vibrio sp
  • Strain
    • UBA10737
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio

Accessions

  • Primary accession
    A0A3D1Z6R9

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-119S1 motif
Region403-406Required for zinc-mediated homotetramerization and catalytic activity
Region530-558Disordered
Region578-777Disordered
Compositional bias599-632Basic and acidic residues
Compositional bias728-744Basic and acidic residues
Compositional bias745-766Basic residues
Region837-862Disordered
Region942-965Disordered

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    965
  • Mass (Da)
    107,107
  • Last updated
    2019-01-16 v1
  • Checksum
    1A1819CAE6F9F1F6
MKRMLINATQKEELRVALVDGQKLFDLDIESPGHESKKANIYKGRITRVEPSLEAAFVDYGADRHGFLPLKEIARDYFPAGYTYQGRPNIKEVLKEGQEVIVQVEKEERGQKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERTQLKAALSTLELPQGMGLIVRTAGVGKSAEELEWDLNILLKHWSAIKEASDSEAAPFLIHQESNVIVRAIRDYLRRDIGEILIDSNTIYERAKAHIQLVRPDFVSRIKKYDGDVPLFSHYQIESQIESAFQREVRLPSGGSIVIDPTEALTSIDINSARATKGGDIEETALNTNLEAADEIARQLRLRDLGGLVVIDFIDMTPVRHQREVETRMREAVRMDRARVQIGRISRFGLLEMSRQRLSPSLAEASHHICPRCTGTGVVRDNESLALSVLRLIEEEALKDNSSQVLAVVPVPIASYLLNEKRKSIQHIEKHQEVNIIVVPNADMETPHFEVVRLRDGEEHQILSYLMPKRIEAFKEAEEPALADMKPRRIEEPVLKGFAPSSAPAPTPTAAPTPAPKAETQAKAKSGGLGGLFKAIGNFLFSGDEPKEEKATEEAKPAQQNRTSNDQRDNNRRRNNRDRNERNNDQRNKRRKPRTNDTADKVAEPAAPQNDVRNDKKRKPKQDKKPAKNATKVVEQGQKIAAEAQAKTTSAEEKTTQVKQRRQRRQMKKKVRVNEQVAEEANVQDLAKADQAEVAAKPEATDKEEGQQRRNRRSPRHLRASGQRRRRTRDRRPNPYRLRAGGVASPEMAMGKVWPQYVAEKPKQKEEQAQPAVETSIPTGGYAFPEMSMGKIVILSKALTETTTTTEAATTQQPVKAEPAPVVETAPVTPTVTEAPAIEATAEVKAEQQDNLVVENTPKAQQAKTPKSVKVKAAKAKSHVSAPMAKASGTGELNNITIIAAPFRESTFTGKAAGSQSETGHASSEMSKTLND

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias599-632Basic and acidic residues
Compositional bias728-744Basic and acidic residues
Compositional bias745-766Basic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DPFO01000052
EMBL· GenBank· DDBJ
HCH02215.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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