A0A3B0MQW6 · A0A3B0MQW6_9RHOB
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids382 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 31 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 71 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 176 | K+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 176-181 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NAGKSS | ||||||
Binding site | 180 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 195 | K+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 195-201 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SDIAGTT | ||||||
Binding site | 197 | K+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 200 | K+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 201 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 220-223 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAG | ||||||
Binding site | 382 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Paracoccaceae > Roseibaca
Accessions
- Primary accessionA0A3B0MQW6
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 166-305 | TrmE-type G | ||||
Sequence: GFEVALVGPVNAGKSSLLNALAGREAAITSDIAGTTRDVLEVRMDVGGYAVTFLDTAGLRDTAEQVEAIGIARGRARAEQADLRIILQPADYIGTVSPSDPQDIVVRSKSDLGPASDDGISVVTGAGLDWLMAEVTNRLS |
Sequence similarities
Family and domain databases
Sequence
- Sequence statusComplete
- Length382
- Mass (Da)40,289
- Last updated2018-12-05 v1
- Checksum6800F98409362FBB
Keywords
- Technical term