A0A3A3KD82 · A0A3A3KD82_SALMO

  • Protein
    Siroheme synthase
  • Gene
    cobA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.

Catalytic activity

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site22-23NAD+ (UniProtKB | ChEBI)
Binding site43-44NAD+ (UniProtKB | ChEBI)
Binding site225S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site248Proton acceptor
Active site270Proton donor
Binding site301-303S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site306S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site331-332S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site382S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site411S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionNAD binding
Molecular Functionprecorrin-2 dehydrogenase activity
Molecular Functionsirohydrochlorin ferrochelatase activity
Molecular Functionuroporphyrin-III C-methyltransferase activity
Biological Processcobalamin biosynthetic process
Biological Processmethylation
Biological Processsiroheme biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Siroheme synthase

Including 3 domains:

  • Recommended name
    Uroporphyrinogen-III C-methyltransferase
  • EC number
  • Short names
    Urogen III methylase
  • Alternative names
    • SUMT
    • Uroporphyrinogen III methylase
      (UROM
      )
  • Recommended name
    Precorrin-2 dehydrogenase
  • EC number
  • Recommended name
    Sirohydrochlorin ferrochelatase
  • EC number

Gene names

    • Name
      cobA
    • Synonyms
      cysG
    • ORF names
      AHY82_05955
      , AIG37_12450
      , APO85_22565
      , AU259_12670
      , AVH73_20640
      , AXU14_19530
      , AXU32_12220
      , B0D32_12790
      , B5881_01010
      , B6C67_06015
      , B6F82_01695
      , BIW02_13375
      , BS131_20695
      , BZ049_13945
      , C2689_15270
      , CW117_11520
      , D0215_03540
      , D2K49_02285
      , D9U82_00785
      , DK664_00780
      , DPE74_06105
      , DPL19_03830
      , DQS63_22775
      , DT992_02860
      , DYO72_17690
      , DYT96_08350
      , E0U36_04570
      , E1406_10255
      , E4933_11310
      , E4S88_10635
      , EIC46_01300
      , FEH78_05450
      , FJJ53_08865
      , G2800_22205
      , G2805_22200
      , G2893_22775
      , G2898_19095
      , G2910_08000
      , G2911_15745
      , G3401_001371
      , G4B33_000853
      , G4Y05_003679
      , G4Y21_000153
      , G4Y22_002812
      , G9254_000510
      , G9G40_000203
      , G9G43_003152
      , G9X30_000953
      , GBH27_09500
      , GND10_002434
      , II32_05055

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 56964
    • 160770
    • 197101
    • 319650
    • 401701
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella

Accessions

  • Primary accession
    A0A3A3KD82

Proteomes

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue128Phosphoserine

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-204Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase
Domain119-145Siroheme synthase central
Domain150-207Sirohaem synthase dimerisation
Region216-457Uroporphyrinogen-III C-methyltransferase
Domain218-426Tetrapyrrole methylase

Sequence similarities

Belongs to the precorrin methyltransferase family.
In the C-terminal section; belongs to the precorrin methyltransferase family.
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    457
  • Mass (Da)
    50,128
  • Last updated
    2018-12-05 v1
  • Checksum
    5B2A57B48D62A3AF
MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLEAGARLTVNALTFIPQFTVWANEGMLTLVEGPFDETLLDSCWLAIAATDDDTVNQRVSEAAESRRIFCNVVDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPQHLGQVARYAGQLRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAVNATTEHLFSEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCHAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQEKLIAFGMQADMPVALVENGTSVKQRVVHGVLTQLGELAQQVESPALIIVGRVVGLRDKLNWFSNH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAFKBF010000006
EMBL· GenBank· DDBJ
EBG8086904.1
EMBL· GenBank· DDBJ
Genomic DNA
AAGEDJ010000003
EMBL· GenBank· DDBJ
EBM9175818.1
EMBL· GenBank· DDBJ
Genomic DNA
AAGJVW010000001
EMBL· GenBank· DDBJ
EBO8585431.1
EMBL· GenBank· DDBJ
Genomic DNA
AAGJXF010000008
EMBL· GenBank· DDBJ
EBO8724379.1
EMBL· GenBank· DDBJ
Genomic DNA
AAGVOP010000264
EMBL· GenBank· DDBJ
EBS4622864.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHIBZ010000005
EMBL· GenBank· DDBJ
EBW3454518.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHQUA010000001
EMBL· GenBank· DDBJ
EBZ3293249.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHYIA010000002
EMBL· GenBank· DDBJ
ECB6706091.1
EMBL· GenBank· DDBJ
Genomic DNA
AAIOTV010000008
EMBL· GenBank· DDBJ
ECG5541193.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKKUG010000004
EMBL· GenBank· DDBJ
ECS8999704.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKLCW010000001
EMBL· GenBank· DDBJ
ECS9166961.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKMKW010000002
EMBL· GenBank· DDBJ
ECT3325251.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPPR010000007
EMBL· GenBank· DDBJ
ECU3335379.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKPRQ010000007
EMBL· GenBank· DDBJ
ECU3685001.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKSUE010000003
EMBL· GenBank· DDBJ
ECV0718804.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKSJB010000002
EMBL· GenBank· DDBJ
ECV2332036.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKSCW010000003
EMBL· GenBank· DDBJ
ECV3149336.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKUXP010000005
EMBL· GenBank· DDBJ
ECV9755739.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKXFP010000003
EMBL· GenBank· DDBJ
ECW6425007.1
EMBL· GenBank· DDBJ
Genomic DNA
AAKYVF010000039
EMBL· GenBank· DDBJ
ECX1556874.1
EMBL· GenBank· DDBJ
Genomic DNA
AALCUX010000007
EMBL· GenBank· DDBJ
ECY3344894.1
EMBL· GenBank· DDBJ
Genomic DNA
AALEDO010000008
EMBL· GenBank· DDBJ
ECY6787821.1
EMBL· GenBank· DDBJ
Genomic DNA
AALGFK010000042
EMBL· GenBank· DDBJ
ECY9608729.1
EMBL· GenBank· DDBJ
Genomic DNA
AALHUF010000006
EMBL· GenBank· DDBJ
ECZ7803670.1
EMBL· GenBank· DDBJ
Genomic DNA
AALIOX010000018
EMBL· GenBank· DDBJ
EDA0012506.1
EMBL· GenBank· DDBJ
Genomic DNA
AALNLT010000029
EMBL· GenBank· DDBJ
EDB4301317.1
EMBL· GenBank· DDBJ
Genomic DNA
AALRRW010000008
EMBL· GenBank· DDBJ
EDC6720060.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMAPH010000009
EMBL· GenBank· DDBJ
EDF3746908.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMARA010000006
EMBL· GenBank· DDBJ
EDF3868613.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMCON010000007
EMBL· GenBank· DDBJ
EDF9740439.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMCZA010000004
EMBL· GenBank· DDBJ
EDG0896523.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMDCD010000001
EMBL· GenBank· DDBJ
EDG1349773.1
EMBL· GenBank· DDBJ
Genomic DNA
AAMKGK010000033
EMBL· GenBank· DDBJ
EDI2489406.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQRW010000021
EMBL· GenBank· DDBJ
HAE0613076.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQTF010000007
EMBL· GenBank· DDBJ
HAE0767951.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQTK010000053
EMBL· GenBank· DDBJ
HAE0786146.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQTN010000024
EMBL· GenBank· DDBJ
HAE0809426.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQUD010000003
EMBL· GenBank· DDBJ
HAE0880103.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAQWK010000020
EMBL· GenBank· DDBJ
HAE1156762.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARLW010000003
EMBL· GenBank· DDBJ
HAE2971120.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARUM010000003
EMBL· GenBank· DDBJ
HAE3985214.1
EMBL· GenBank· DDBJ
Genomic DNA
DAARVT010000008
EMBL· GenBank· DDBJ
HAE4143879.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATOZ010000001
EMBL· GenBank· DDBJ
HAE8947232.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATOC010000049
EMBL· GenBank· DDBJ
HAE9354060.1
EMBL· GenBank· DDBJ
Genomic DNA
DAATOK010000005
EMBL· GenBank· DDBJ
HAE9389018.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUAZ010000008
EMBL· GenBank· DDBJ
HAF0923478.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAUCY010000001
EMBL· GenBank· DDBJ
HAF1250544.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAWCN010000004
EMBL· GenBank· DDBJ
HAF7251952.1
EMBL· GenBank· DDBJ
Genomic DNA
DAAWDQ010000003
EMBL· GenBank· DDBJ
HAF7388039.1
EMBL· GenBank· DDBJ
Genomic DNA
SRBT01000004
EMBL· GenBank· DDBJ
KAA7153371.1
EMBL· GenBank· DDBJ
Genomic DNA
CP074666
EMBL· GenBank· DDBJ
QVS86478.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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