A0A3A3KD82 · A0A3A3KD82_SALMO
- ProteinSiroheme synthase
- GenecobA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids457 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
Catalytic activity
- siroheme + 2 H+ = sirohydrochlorin + Fe2+
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22-23 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DV | ||||||
Binding site | 43-44 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: LT | ||||||
Binding site | 225 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Active site | 248 | Proton acceptor | ||||
Sequence: D | ||||||
Active site | 270 | Proton donor | ||||
Sequence: K | ||||||
Binding site | 301-303 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: GGD | ||||||
Binding site | 306 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 331-332 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TA | ||||||
Binding site | 382 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 411 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | NAD binding | |
Molecular Function | precorrin-2 dehydrogenase activity | |
Molecular Function | sirohydrochlorin ferrochelatase activity | |
Molecular Function | uroporphyrin-III C-methyltransferase activity | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | methylation | |
Biological Process | siroheme biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSiroheme synthase
Including 3 domains:
- Recommended nameUroporphyrinogen-III C-methyltransferase
- EC number
- Short namesUrogen III methylase
- Alternative names
- Recommended namePrecorrin-2 dehydrogenase
- EC number
- Recommended nameSirohydrochlorin ferrochelatase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionA0A3A3KD82
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 128 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-204 | Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase | ||||
Sequence: MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLEAGARLTVNALTFIPQFTVWANEGMLTLVEGPFDETLLDSCWLAIAATDDDTVNQRVSEAAESRRIFCNVVDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPQHLGQVARYAGQLRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAVNATT | ||||||
Domain | 119-145 | Siroheme synthase central | ||||
Sequence: RSPLMVAVSSGGTSPVLARLLREKLES | ||||||
Domain | 150-207 | Sirohaem synthase dimerisation | ||||
Sequence: HLGQVARYAGQLRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAVNATTEHL | ||||||
Region | 216-457 | Uroporphyrinogen-III C-methyltransferase | ||||
Sequence: GEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCHAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQEKLIAFGMQADMPVALVENGTSVKQRVVHGVLTQLGELAQQVESPALIIVGRVVGLRDKLNWFSNH | ||||||
Domain | 218-426 | Tetrapyrrole methylase | ||||
Sequence: VVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCHAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQEKLIAFGMQADMPVALVENGTSVKQRVVHGVLTQL |
Sequence similarities
Belongs to the precorrin methyltransferase family.
In the C-terminal section; belongs to the precorrin methyltransferase family.
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length457
- Mass (Da)50,128
- Last updated2018-12-05 v1
- Checksum5B2A57B48D62A3AF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFKBF010000006 EMBL· GenBank· DDBJ | EBG8086904.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAGEDJ010000003 EMBL· GenBank· DDBJ | EBM9175818.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAGJVW010000001 EMBL· GenBank· DDBJ | EBO8585431.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAGJXF010000008 EMBL· GenBank· DDBJ | EBO8724379.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAGVOP010000264 EMBL· GenBank· DDBJ | EBS4622864.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHIBZ010000005 EMBL· GenBank· DDBJ | EBW3454518.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHQUA010000001 EMBL· GenBank· DDBJ | EBZ3293249.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHYIA010000002 EMBL· GenBank· DDBJ | ECB6706091.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAIOTV010000008 EMBL· GenBank· DDBJ | ECG5541193.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKKUG010000004 EMBL· GenBank· DDBJ | ECS8999704.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKLCW010000001 EMBL· GenBank· DDBJ | ECS9166961.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKMKW010000002 EMBL· GenBank· DDBJ | ECT3325251.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPPR010000007 EMBL· GenBank· DDBJ | ECU3335379.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKPRQ010000007 EMBL· GenBank· DDBJ | ECU3685001.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKSUE010000003 EMBL· GenBank· DDBJ | ECV0718804.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKSJB010000002 EMBL· GenBank· DDBJ | ECV2332036.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKSCW010000003 EMBL· GenBank· DDBJ | ECV3149336.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKUXP010000005 EMBL· GenBank· DDBJ | ECV9755739.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKXFP010000003 EMBL· GenBank· DDBJ | ECW6425007.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAKYVF010000039 EMBL· GenBank· DDBJ | ECX1556874.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALCUX010000007 EMBL· GenBank· DDBJ | ECY3344894.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALEDO010000008 EMBL· GenBank· DDBJ | ECY6787821.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALGFK010000042 EMBL· GenBank· DDBJ | ECY9608729.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALHUF010000006 EMBL· GenBank· DDBJ | ECZ7803670.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALIOX010000018 EMBL· GenBank· DDBJ | EDA0012506.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALNLT010000029 EMBL· GenBank· DDBJ | EDB4301317.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AALRRW010000008 EMBL· GenBank· DDBJ | EDC6720060.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMAPH010000009 EMBL· GenBank· DDBJ | EDF3746908.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMARA010000006 EMBL· GenBank· DDBJ | EDF3868613.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMCON010000007 EMBL· GenBank· DDBJ | EDF9740439.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMCZA010000004 EMBL· GenBank· DDBJ | EDG0896523.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMDCD010000001 EMBL· GenBank· DDBJ | EDG1349773.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAMKGK010000033 EMBL· GenBank· DDBJ | EDI2489406.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQRW010000021 EMBL· GenBank· DDBJ | HAE0613076.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQTF010000007 EMBL· GenBank· DDBJ | HAE0767951.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQTK010000053 EMBL· GenBank· DDBJ | HAE0786146.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQTN010000024 EMBL· GenBank· DDBJ | HAE0809426.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQUD010000003 EMBL· GenBank· DDBJ | HAE0880103.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAQWK010000020 EMBL· GenBank· DDBJ | HAE1156762.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARLW010000003 EMBL· GenBank· DDBJ | HAE2971120.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARUM010000003 EMBL· GenBank· DDBJ | HAE3985214.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAARVT010000008 EMBL· GenBank· DDBJ | HAE4143879.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATOZ010000001 EMBL· GenBank· DDBJ | HAE8947232.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATOC010000049 EMBL· GenBank· DDBJ | HAE9354060.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAATOK010000005 EMBL· GenBank· DDBJ | HAE9389018.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUAZ010000008 EMBL· GenBank· DDBJ | HAF0923478.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAUCY010000001 EMBL· GenBank· DDBJ | HAF1250544.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAWCN010000004 EMBL· GenBank· DDBJ | HAF7251952.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAAWDQ010000003 EMBL· GenBank· DDBJ | HAF7388039.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SRBT01000004 EMBL· GenBank· DDBJ | KAA7153371.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP074666 EMBL· GenBank· DDBJ | QVS86478.1 EMBL· GenBank· DDBJ | Genomic DNA |