A0A3A1XS06 · A0A3A1XS06_9BIFI
- ProteinThymidylate synthase
- GenethyA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids290 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity
- dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 44 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: R | ||||||
Binding site | 153-154 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: RR | ||||||
Active site | 173 | |||||
Sequence: C | ||||||
Active site | 173 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 193-196 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: RSCD | ||||||
Binding site | 196 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 204 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: N | ||||||
Binding site | 234-236 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: HIY | ||||||
Binding site | 289 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: A |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymidylate synthase
- EC number
- Short namesTS ; TSase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae
Accessions
- Primary accessionA0A3A1XS06
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 26-290 | Thymidylate synthase/dCMP hydroxymethylase | ||||
Sequence: PYEDLVRKILTEGTLKSDRTGTGTISLFGQQIRFDISKYFPLITTKTVFFKGLAYELLWFLKGSSNVRWLQENGVHIWDEWADENGDLGPVYGVQWRSWPASTAENPNRTIDQIANVLDLMKNHPDSRRMIVSAWNPAEVEHMALPPCHSLFQFYVADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQAGLEPGEFVWTGGDCHIYDNHVEQVLEQLSREPYPYPQLEIRKADSIFDYDYSDFKVVEYKHHPTIKAPVAV |
Sequence similarities
Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length290
- Mass (Da)33,172
- Last updated2018-12-05 v1
- ChecksumD4572727594722F9