A0A3A1XS06 · A0A3A1XS06_9BIFI

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site44dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site153-154dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site173
Active site173Nucleophile
Binding site193-196dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site196(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site204dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site234-236dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site289(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      CJI49_04170

Organism names

Accessions

  • Primary accession
    A0A3A1XS06

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain26-290Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    290
  • Mass (Da)
    33,172
  • Last updated
    2018-12-05 v1
  • Checksum
    D4572727594722F9
MALTEEELAAIRARVPQKPLSSIPTPYEDLVRKILTEGTLKSDRTGTGTISLFGQQIRFDISKYFPLITTKTVFFKGLAYELLWFLKGSSNVRWLQENGVHIWDEWADENGDLGPVYGVQWRSWPASTAENPNRTIDQIANVLDLMKNHPDSRRMIVSAWNPAEVEHMALPPCHSLFQFYVADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQAGLEPGEFVWTGGDCHIYDNHVEQVLEQLSREPYPYPQLEIRKADSIFDYDYSDFKVVEYKHHPTIKAPVAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NQOQ01000002
EMBL· GenBank· DDBJ
RIY28763.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp