A0A397HQ89 · ANKC_ASPTH

Function

function

FAD-dependent monooxygenase; part of the ank cluster that mediates the biosynthesis of NK13650 C, a highly modified cyclo-arginine-tyrosine dipeptide (PubMed:36702957).
AnkC uses as substrate the dehydro-cyclodipeptide intermediate generated by the monooxygase ankB and acts as a hydroxylase that installs the m-OH through a canonical flavin-dependent aromatic hydroxylation mechanism (PubMed:36702957).
Within the pathway, the cyclodipeptide synthase ankA acts as the scaffold-generating enzyme and is responsible for formation of the cyclo-Arg-Tyr diketopiperazine (cRY) from L-Arg and L-Tyr. The ankA product cRY is desaturated by the cytochrome P450 monooxygenase ankB to yield a dehydro-cyclodipeptide intermediate. The FAD-dependent monooxygenase ankC then installs the m-OH, ankD catalyzes the attachment of L-homoserine, and ankE ligates citrate to the ankD product to yield NK13650 B. The O-methyltransferase ankF is responsible for methylation of the C-17 phenol group of NK13650 B to produce NK13650 D. Amidation of NK13650 D with L-Asp by ankG then leads to the production of NK13650 C, whereas amidation of NK13650 B produces NK13650 A (PubMed:36702957).

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site245FAD (UniProtKB | ChEBI)
Binding site311FAD (UniProtKB | ChEBI)

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent monooxygenase ankC
  • EC number
  • Alternative names
    • Ank biosynthesis cluster protein C

Gene names

    • Name
      ankC
    • ORF names
      CDV56_109012

Organism names

Accessions

  • Primary accession
    A0A397HQ89

Proteomes

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane7-27Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004606561-587FAD-dependent monooxygenase ankC

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the PheA/TfdB FAD monooxygenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    587
  • Mass (Da)
    65,803
  • Last updated
    2018-12-05 v1
  • Checksum
    0678F7F0C95B0FE6
MTKKESAVDVLIIGAGPAGLIAAMWMAKCGITTRVVEQRPYQLRVGGADGIHPRSMEMFESFGIDQEITQVWEPITGWALWCRNAEGTLARSDRMDNPTPVRCRWGPGLLQQGIIERIIKEHISEQPSVRIEHETVSASLVIMEDALDKPDSHPCVITLRHDDSAGGSEELVRAKYVIGADGARSWTRKQLGFKMEGTRTRSVWAVTDLVVVSDFPDIRLCTTINSYGEGGLFFLRRERGLTRFYVQLNRADEVEFPAASITQELIIERLQRLLRPYTLTVKRCEWWSSYTVAHYLSDGMTKHDRVFLVGDAVHNHSPLVGLGMNISMQDSYNLGWKLAGVLKKELNPSILSTYETERRPVAAELIETDRFHLQLFDTATVTGSEPAWMLEREEALQPSMQGFAVHYQDPLLTVATEKECRPDAVIPGKRFPQLNVSNHATGKVYSIQSLLKSKGKFHVIVFAGDLSQPLELNRFNTCGTALQQIEEQILPASMGKFNVIAVHRARKTAIELASLADIFFPVDETTGRDYNRVYCDMETSYEEAGIGEQGAVVLVRPDQYVGWCGEVEDVQGLTGYLQPIFEAKKHA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NKHU02000029
EMBL· GenBank· DDBJ
RHZ63464.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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