A0A386KZI3 · DABD_PSEMU
- ProteinN-geranyl-L-glutamate oxidase
- GenedabD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids559 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cytochrome P450 monooxygenase: part of the gene cluster that mediates the biosynthesis of domoic acid (DA) and derivatives, natural products with neurochemical activity acting as ionotropic glutamate receptor (iGluR) agonists, thus being neurotoxins causing amnesic shellfish poisoning (ASP) (PubMed:30262498).
Catalyzes the conversion of N-geranyl-L-glutamic acid (L-NGG) to N-(7'-carboxy-7'-demethylgeranyl)-L-glutamic acid (cNGG) via three successive steps involving N-(8'-hydroxygeranyl)-L-glutamic acid and N-(8'-oxogeranyl)-L-glutamic acid as intermediates (PubMed:30262498).
Catalyzes the conversion of N-geranyl-L-glutamic acid (L-NGG) to N-(7'-carboxy-7'-demethylgeranyl)-L-glutamic acid (cNGG) via three successive steps involving N-(8'-hydroxygeranyl)-L-glutamic acid and N-(8'-oxogeranyl)-L-glutamic acid as intermediates (PubMed:30262498).
Catalytic activity
- N-geranyl-L-glutamate + O2 + reduced [NADPH--hemoprotein reductase] = H+ + H2O + N-(8'-hydroxygeranyl)-L-glutamate + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- N-(8'-hydroxygeranyl)-L-glutamate + O2 + reduced [NADPH--hemoprotein reductase] = H+ + 2 H2O + N-(8'-oxogeranyl)-L-glutamate + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- N-(8'-oxogeranyl)-L-glutamate + O2 + reduced [NADPH--hemoprotein reductase] = 2 H+ + H2O + N-(7'-carboxy-7'-demethylgeranyl)-L-glutamate + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | cellular response to carbon dioxide | |
Biological Process | cellular response to phosphate starvation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-geranyl-L-glutamate oxidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Ochrophyta > Bacillariophyta > Bacillariophyceae > Bacillariophycidae > Bacillariales > Bacillariaceae > Pseudo-nitzschia
Accessions
- Primary accessionA0A386KZI3
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 49-71 | Helical | ||||
Sequence: AVACASLGFVMYCVLSFARSYYR |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454275 | 1-559 | N-geranyl-L-glutamate oxidase | |||
Sequence: MNIATASLCAGLAVSGKFLSKYYQFLSTSQSDEVGFIEIMNMGMTGNDAVACASLGFVMYCVLSFARSYYRFRFSPLRNAPGFGPKSFVYGMFYEFLEAPFMEPPIEALKKLRKGGNDIPFLAYTTLFGSQRLLLLDCDLIKQVFTAPSGRDPMRYPKHYVYLREVVGDGLVVVEGSEWSRHRRIVQPAFQSAFLRDAITTTVPTLVENLVDVWKKTAGTSINLNAHLSIITLDVIGKVAFSHEFNASKLLNEWAECPDKELGEVDDPLISSIGAAFSSSPLKLMLTVLKQPWLEKHLSPAFRTSRNLLNKAADDIVQNAKNIDDPKRRSVLNLMMEAKDDESSKARNKLTDTELRDEVKTFLVAGHETTSTWAHWSLYVLATRPDIQEKVFADITKHSPDDKTILLEQADQMEYLWAFMNETLRLYSPLGLISRVTHKEENFKGYTIPAGTNLRIPIHLIHRHPDHWKDPEDFKPERWFDTEESSKRHKFAFIPFAAGGRNCIGQRFASMEAKIIVANVAKNFEIHLADSMKGKKITFSNFISLKCKPEIEIYVTPRG |
Expression
Induction
Up-regulated under phosphate limitation and by increasing partial pressure of CO2.
Structure
Sequence
- Sequence statusComplete
- Length559
- Mass (Da)63,266
- Last updated2018-12-05 v1
- ChecksumEAB402B04A884DA2