A0A386JTF7 · A0A386JTF7_9NIDO

Function

function

Cleaves the majority of cleavage sites present in the C-terminus of the polyprotein. Triggers host apoptosis through caspase-3, -8, and -9 activations. Subverts host innate immune responses through its protease activity. Targets the NF-kappa-B essential modulator NEMO and mediates its cleavage. Blocks host interferon beta induction and downstream signaling by cleaving mitochondrial MAVS, dislodging it from the mitochondria. Impairs host defense by cleaving host mRNA-decapping enzyme DCP1A to attenuate its antiviral activity.
Contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
Displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.
Plays a role in the inhibition of host STAT3 signaling pathway by inducing the degradation of STAT3.
Plays a role in the inhibition of the immune response by interacting with host IFITM1. This interaction leads to the proteasomal degradation of the IFN-induced antiviral protein IFITM1.
Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic.
Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, PKR (By similarity) and NLRP3 inflammasome (By similarity).
Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity).
If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors (By similarity).
Also plays a role in the inhibition of host type I interferon production by recruiting host OTULIN to promote removal of linear ubiquitination targeting host NEMO
Responsible for replication and transcription of the viral RNA genome.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site3613
Active site3628
Active site3657

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum
Cellular Componenthost cell membrane
Cellular Componenthost cell nucleus
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular Functionprotein serine/threonine kinase inhibitor activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA nuclease activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionserine-type endopeptidase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processproteolysis
Biological Processsymbiont-mediated suppression of host ISG15-protein conjugation
Biological Processsymbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity
Biological Processsymbiont-mediated suppression of host NF-kappaB cascade
Biological Processsymbiont-mediated suppression of host PKR/eIFalpha signaling
Biological Processsymbiont-mediated suppression of host type I interferon-mediated signaling pathway
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Replicase polyprotein 1ab
  • Alternative names
    • ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • IA14737-2016
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Arnidovirineae > Arteriviridae > Variarterivirinae > Betaarterivirus > Ampobartevirus > Betaarterivirus suid 2

Accessions

  • Primary accession
    A0A386JTF7

Subcellular Location

Cytoplasm
Endoplasmic reticulum
Host endoplasmic reticulum
Host membrane
; Multi-pass membrane protein
Host nucleus
Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1551-1573Helical
Transmembrane1585-1609Helical
Transmembrane1621-1647Helical
Transmembrane1921-1947Helical
Transmembrane1959-1980Helical
Transmembrane1992-2012Helical
Transmembrane2032-2057Helical
Transmembrane2064-2082Helical

Keywords

Interaction

Subunit

Interacts with host DDX18; this interaction redistributes host DDX18 to the cytoplasm.
Interacts with host DDX5.
Interacts with host IFITM1.
Interacts with host LGALS3.
Interacts with host OTULIN.
Nsp1-alpha papain-like: Interacts with host RNF31.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain69-180Peptidase C31
Domain263-383Peptidase C32
Region387-417Disordered
Domain428-535Peptidase C33
Region702-782Disordered
Compositional bias725-741Basic and acidic residues
Compositional bias853-867Polar residues
Region853-875Disordered
Region1049-1112Disordered
Compositional bias1075-1089Polar residues
Domain1285-13164Fe-4S ferredoxin-type
Domain1710-1913Peptidase S32
Domain2388-2550NiRAN
Domain2789-2923RdRp catalytic
Domain3044-3107AV ZBD
Domain3164-3445+RNA virus helicase C-terminal
Domain3484-3580AV-Nsp11N/CoV-Nsp15M
Domain3582-3704NendoU

Sequence similarities

Belongs to the arteriviridae polyprotein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,860
  • Mass (Da)
    421,613
  • Last updated
    2018-12-05 v1
  • Checksum
    BC063D50739AC741
MSGILDRCTCTPNARVFMAEGQVYCTRCLSARSLLPLNLQVSELGVLGLFYRPEEPLRWTLPRAFPTVECSPAGACWLSAIFPIARMTSGNLNFQQRLVRVAAEIYRAGQLTPAVLKNLQVYERGCRWYPIVGPVPGVGVYANSLHVSDRPFPGATHVLTNLPLPQKPKPEDFCPFECAMAAVYDIGHDAVMFVAEGRVSWAPRGGDKGRFEIVPEGLRLIAEQLHASFPPYHMVDVSEFSFVAPECGASMRVERQYGCLPADTVPEGNCWWSLFSSLPWKVQYEEIRRASQFGYQTKHGVAGKYLQRRMQVNGLRAVTDTSGPIVVQYFCVKESWIRHLRLAEEPSLPGFEDLIRIRVEPNTSPLVGEGEKIFRFGTHKWYGAGKRTRKARSGAATTGTRRVSPVRETQQAKEHEDISANKAEQLEHYSPPADGNCGWHCISAIINHLVNSKLETTLPERVRPSDDWATDENLVNAIQTLRLPVSLDRNGACVGARYVLKLEGEHWTVSVAPGVTPLLLPLECVQGCCEHKSGLGPSDVVEFSGLDPTCSDRLAGVMHLPSCVIPDALAEMSGDPNCPASPIATVWTTSQFYAHYRGGEHPDQVCLRKIISLCQVLEECCCSQSKTHRVTPEEVAAKIDSYLRGAANLDECLARLEKARPPSVMDASLNWNVVFPGVEAGVHLTELPPANQCRAPTSIATQGLLEDGPAPSPPLTPRVQPRKTRSVRSLPDCKPIPAPRRRIRSDCGGPILMGDNVPDGREDLTVSGPSDLPTPSELMTPPSEPMFVPASRHTSKPVAFLNRLAPVPAPRKIVSRQTVPSSESILASTQRHDFQQVNGANLTATMLTCQDEPLDSSSSSLTEYEASPLGPLQNVDIPGTGEQEAEEVLNGIPDILDDANPASVSSSSSLSSVEITRPKYSAQAIIDTGGPCCGHLREVKEKYLNVMREACDATKLNDPATQEWLSRMWDRVDMLTWRNTSIFQAPFTLADKFKSLPKMILETPPPYPCGFVMMPRTPAPSVGAESDLTVGSVATEDVPYILGREQGVGKATDQGPLAPFAGELTDGQPAREPRTQAPSACTSGVGLVLDSGSSLEPADLPPPNGTDAGGGGPLYTVKKKAEKCFDQLSQRVFGIVSHLPVFLSRLFRPDGHYSPGDWGFAAFTLLCLFLCYSYPAFGAAPLLGVFSGSSRRVRMGVFGCWLAFAVSLFKPAPDPVGTACEFDSPECRDILHSFELLQPWDPVRSLVVGPVGLCLAVLGRLLGGARYVWLLLLRLGILSDCVLAGAYVLSQGRCKKCWGSCVRTAPSEVAFNVFPFTRATRSSLIDLCNRFCAPKGMDPIFLATGWRGCWTGQSPIEQSCEKPIAYAQLDEKKITARTVVAQPYDPNQAVKCLRVLQAGGVMVAEAVPKVTKVSVIPFRAPFFPTGVKVDPDCRIVVDPDTFTTALRSGYSTSNLVLGVGDFAQLNGLKIRQISKPSGGGPHLAAALHVACSMALHMLAGIYVTSVGTCGTGTNDPWCTNPFAVPGYGPGSLCTSRLCISQHGLTLPLTTLVAGFGIQEIALVVLIFVSIGGLAHRLSCKADVMFVILAIASYVWEPLTWLLCVFPCWLRCFSLHPLTILWLVFFLISVNMPSGILALVLLVSLWLLGRYTNVAGLVTPYDIHHYTNGPRGVAALATAPDGTYLAAVRRAALTGRTMLFTPSQLGSLLEGAFRTQKPSLNTVNVVGSSMGSGGVFTIDGKIKCVTAAHVLTGNSARVSGVGFNQMLDFDVRGDFAIADCPNWHGAAPKTQFCADEWTGRAYWLTSSGVEPGVIGKGFAFCFTACGDSGSPVITEAGELVGIHTGSNKQGGGIVTRPSGQLCSVAPVKLSELSEFFAGPKVPLGDVKVGSHIIKDISEVPSDLCALLAAKPELEGGLSTVQLLCVFFLLWRMMGHAWTPLVAVSFFILNEVLPAVLVRSVFSFGMFVLSWLTPWSAQVLMIRLLTAALNRNRLSLAFFSLGAVTGFVADLATTQGHPLQTVMNLSTYAFLPRMVVVTSPVPVIACGVVHLLAIILYLFKYRGLHHILVGDGVFSAAFFLRYFAEGKLREGVSQSCGMNHESLTGALAMKLNDEDLDFLTKLTDFKCFVSASNMRNAAGQFIEAAYAKALRVELAQLVQVDKVRGVLAKLEAFADTAMPQLSPGDIVVALGHTPVGSIFDLKVGSTKHTLQAIETRVLAGSRMTVARVVDPAPAPPPVPVPIPLPSKVLENGPRAWEDEDRLNKKKRRRMEAVGIYVMDGKKYQKFWDKNSGDVFYEEVHDNTDAWECLRTGDPADFDPEKGTLCGRLTIENKPYNVYTSPSGRKFLVPANPESGKVQWEAARLSMEQALGMMNVDGELTAKEVEKLKRIIDKLQGLTREQCLNYLAASGLTRCGRGGLVVTETAVKIVKFHNRTFTLGPVNLKVASEVELKDAVEHNQHPVAKPVDGGVVLLRSAVPSLVDVLISGADASPKLIARHGPGNTGIDGTLWDFEAEATKEEIALSAQIIQACDIRRGDAPEIGLPYKLHPVRGNPERVKGVLKNTRFGDIPYKTPSDTGSPVHAAACLTPNATPVTDGRSVLATTMPSGFELYVPTIPASVLDYLDSRPDCPKQLTEHGCEDAALRDLSKYDLSTQGFVLPGVLRLVRKYLFAHVGKCPPVHRPSTYPAKNSMAGINGNRFPTKDIQSIPEIDVLCAQAVRENWQTVTPCTLKKQYCGKKKTRTILGTNNFIALAHRAALSGVTQGFMKKAFNSPIALGKNKFKELQTPVLGRCLEADLASCDRSTPAIVRWFAAHLLYELACAEEHLPSYVLNCCHDLLITQSGAVTKRGGLSSGDPITSVSNTIYSLVIYAQHMVLSYFKSGHPHGLLFLQDQLKFEDMLKVQPLIVYSDDLVLYAESPTMPNYHWWVEHLNLMLGFQTDPKKTTITDSPSFLGCRIMSGRQLVPNRDRILAALAYHMKANNVSEYYASAAAILMDSCACLEYDPEWFEELVVGMAQCARKDGYSFPGPPFFLSMWEKLRSNYEGKKSRVCGYCGASAPYATACGLDVCVYHTHFHQHCPVIIWCGHPAGSGSCDECKSPIGKGTSPLDEVLRQVPYKPPRTVLMHVEQGLTPLDPGRYQTRRGLVAVRRGIRGNEVDLPDGDYASTALLPTCKEINMVAVASNVLRSRFIIGPPGAGKTHWLLQQVQDGDVIYTPTHQTMLDMIKALGTCRFNVPVGTTLQFPAPSRTGPWVRILAGGWCPGKNSFLDEAAYCNHLDVLRLLSKTTLTCLGDFKQLHPVGFDSHCYVFDIMPQTQLKTIWRFGQNICDAIQPDYRDKLMSMVNTTRVTYVEKPVNYGQVLTPYHRDREDSAITIDSSQGATFDVVTLHLPTKDSLNKQRALVAITRARHAIFVYDPHGQLQSLFDLPAKSTPVNLAVLRDGQLIVLDRNNKECTVAQALGNGDKFRATDKRVVDSLRAICADLEGSSSPLPKVAHNLGFYFSPDLTQFAKLPIELAPHWPVVTTQNNENWPDRLVASLRPIHKYSRACIGAGYMVGPSVFLGAPGVVSYYLTKFVKGEAQVLPETIFSTGRIEVDCREYLDDREREVAASLPHAFIGDVKGTTVGGCHHVTSKYLPRFLPKESVAVVGVSSPGKAAKAVCTLTDVYLPDLEAYLHPVTQSKCWKMMLDFKEVRLMVWKDRTAYFQLEGRHFTWYQLASFASYIRVPVNSTVYLDPCMGPALCNRKVVGSPHWGADLAVTPYDYGARKILSSAYHGEMPPGYKILACAEFSLDDPVRYRHTWGFESDTAYLYEFTGNGEDWEDYNDAFRARQKGKIYKATATSLKFHFPPGHIVEPTLGLN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias725-741Basic and acidic residues
Compositional bias853-867Polar residues
Compositional bias1075-1089Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF663706
EMBL· GenBank· DDBJ
AYD75404.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp