A0A384JK99 · PTF_BOTFB
- ProteinAromatic prenyltransferase
- Geneptf
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids309 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score2/5
Function
function
Prenyltransferase that attaches isoprenoid moieties to carbon atoms of aromatic substrates in an enzyme-catalyzed Friedel-Crafts reaction (PubMed:20351110).
Shows specificity for dimethylallyl diphosphate (DMAPP) and does not accept geranyl diphosphate (GPP) or isopentenyl diphosphate (IPP) (PubMed:20351110).
Prenylates the artificial substrate 2,7-dihydroxynaphthalene (2,7-DHN), as well as dihydrophenazine-1-carboxylic acid and 4-hydroxybenzoic acid at lower levels (PubMed:20351110).
Only traces of products are detected with aspulvinone E or flaviolin as substrates; and no product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate (PubMed:20351110).
Ptf seems no to be involved in the prenylation reaction in the biosynthesis of aspulvinone H and J and the physiological function of ptf remains unknown (PubMed:20351110).
Shows specificity for dimethylallyl diphosphate (DMAPP) and does not accept geranyl diphosphate (GPP) or isopentenyl diphosphate (IPP) (PubMed:20351110).
Prenylates the artificial substrate 2,7-dihydroxynaphthalene (2,7-DHN), as well as dihydrophenazine-1-carboxylic acid and 4-hydroxybenzoic acid at lower levels (PubMed:20351110).
Only traces of products are detected with aspulvinone E or flaviolin as substrates; and no product is formed with L-tryptophan, L-tyrosine, or 4-hydroxyphenylpyruvate (PubMed:20351110).
Ptf seems no to be involved in the prenylation reaction in the biosynthesis of aspulvinone H and J and the physiological function of ptf remains unknown (PubMed:20351110).
Miscellaneous
The gene is not located within a recognizable secondary metabolic gene cluster.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1010 μM | DMAPP | |||||
996 μM | 2,7-dihydroxynaphthalene |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | prenyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAromatic prenyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Helotiales > Sclerotiniaceae > Botrytis
Accessions
- Primary accessionA0A384JK99
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000455462 | 1-309 | Aromatic prenyltransferase | |||
Sequence: MVVQAVSESLKFDQSRFLNDIQILSAAINAPYSESTTKKVLSVFSDSFHDGVVLWRATDRVGDALNYRFYSRRPIDTVTIASSAGLLSPDVVSNLGRLVTSWSSLYDGLPEESCDFDAEKGLVKAWVYMRGMRPLGDILSAEGVPESLKQHEERFKALELEKVRHVAVDYQKATVNLYFRAQGPISLQQATSFNALAGAGPPSQTQFLEMQEFLNAVGYTFAVTIRVDSGDIERVGYYALKLPDRATKNWPVINAQLEKFAQFAPSYDREEMNAVAWSFGGTKRYVKFERSYCGELVPLIKGWGTTLSS |
Structure
Sequence
- Sequence statusComplete
- Length309
- Mass (Da)34,474
- Last updated2018-11-07 v1
- Checksum434F9267B029F90A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP009810 EMBL· GenBank· DDBJ | ATZ50774.1 EMBL· GenBank· DDBJ | Genomic DNA |