A0A373FGU6 · A0A373FGU6_COMTE
- ProteintRNA (cytidine(34)-2'-O)-methyltransferase
- GenetrmL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids164 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
Catalytic activity
- 5-carboxymethylaminomethyluridine34 in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu) + S-adenosyl-L-homocysteine + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 80 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 102 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 124 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 132 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | RNA binding | |
Molecular Function | tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (cytidine(34)-2'-O)-methyltransferase activity | |
Biological Process | wobble position cytosine ribose methylation | |
Biological Process | wobble position uridine ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Comamonas
Accessions
- Primary accessionA0A373FGU6
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-143 | tRNA/rRNA methyltransferase SpoU type | |||
Sequence similarities
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length164
- Mass (Da)18,177
- Last updated2018-11-07 v1
- Checksum12048DFFFF79A994
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QURR01000019 EMBL· GenBank· DDBJ | RGE43343.1 EMBL· GenBank· DDBJ | Genomic DNA |