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A0A371MBP4 · A0A371MBP4_9EURY

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

Type
IDPosition(s)Description
Site52Interaction with DNA
Site165Interaction with DNA
Site169Interaction with DNA
Active site326O-(5'-phospho-DNA)-tyrosine intermediate
Site328Interaction with DNA
Site515Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      C5B91_01895

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Atlit-10N
  • Taxonomic lineage
    Archaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Haloferacaceae > Haloferax

Accessions

  • Primary accession
    A0A371MBP4

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain5-140Toprim
Region193-198Interaction with DNA
Region359-392Disordered

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    840
  • Mass (Da)
    93,578
  • Last updated
    2018-11-07 v1
  • MD5 Checksum
    7A816D5C29CF16BE6DD2ED11401F2326
MSRGPELIITEKDNAARRIADILSGETASAERMNGVNVYKWGGKRCIGLSGHVVGVDFPPEYNDWRDVEPVELIGAPVEKHPTQENIVAALRRLARRAGTVTIATDYDREGELIGKEAYELVRDVNEDVPVDRVRFSSITKREVTEAFDNPDDLDFNLAAAGEARQIIDLVWGAALTRFLSLSARQLGNDFISVGRVQGPTLKLIVDREREIEAFDPEDYWELFSKLTKDADGDAESFEAQYFYLDDDGTEAERIWDEESAESAYQTLLGVEAAEVASVNRRTRTDTPPAPFNTTQFIRAAGSIGYSAQRAMSIAEDLYTAGYITYPRTDNTVYPDDLDPRELLGAFEGDRRFKEDAESLLEQEEIEPTEGDNETTDHPPIHPTGELPSASDLTEDEWDVYELVVRRFFATVAEDAVWEHLRVVAEVEGLSLKANGKRLLEAGYHDVYPYFNSSESFVPDVEEGESLVLSDTHLDAKQTQPPRRYGQSRLIETMEQMGIGTKATRHDVIQKLYDRGYIESDPPRPTRLARAVVEASEDFAKLIVSEEMTSQLESDMLAIARGEATLEDVTEESKEMLQDVFEGLMESREELGKQLQDSLKADKTVGTCPDCGGDLVVRKSRRGSYFIGCDSYPDCTYTLPLPSTGKPLIMEDSCEEHDLHHIKMLAGRKTFVHGCPLCKAEEADEEDDLVIGTCPECGEEEGGELAIKRLRSGSRLVGCTRYPDCDYSLPLPRRGDIEVTDESCEEHDLPELRITYEGDREPWELGCPICNYREYQAQQNGEGGSDLESISGIGAKTAEKLKDAGIEDVKTLKASEPDDIAAKVEGVGADTVRKWQTAAD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PSYX01000001
EMBL· GenBank· DDBJ
RDZ60281.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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