A0A370PCS5 · A0A370PCS5_ASPPH

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of DRE2, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. Positively controls H2O2-induced cell death.
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site19-24FMN (UniProtKB | ChEBI)
Binding site66-69FMN (UniProtKB | ChEBI)
Binding site104-113FMN (UniProtKB | ChEBI)
Binding site357FAD (UniProtKB | ChEBI)
Binding site387-390FAD (UniProtKB | ChEBI)
Binding site426-429FAD (UniProtKB | ChEBI)
Binding site468NADP+ (UniProtKB | ChEBI)
Binding site536-537NADP+ (UniProtKB | ChEBI)
Binding site542-546NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrial matrix
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      TAH18
    • ORF names
      M752DRAFT_285381

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 13157
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus

Accessions

  • Primary accession
    A0A370PCS5

Proteomes

Subcellular Location

Cytoplasm
Mitochondrion
Mitochondrion matrix
Note: Relocalizes to mitochondria after H2O2 exposure.

Keywords

Interaction

Subunit

Interacts with DRE2; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain13-162Flavodoxin-like
Region168-198Disordered
Domain202-456FAD-binding FR-type

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    889
  • Mass (Da)
    98,485
  • Last updated
    2018-11-07 v1
  • Checksum
    74D24A29B4AD9892
MDGSPSTPPRRSALVLYGSETGNSQEVAEELGALAERLHFHTHVGEMNEYRPEVLKSHTLVIFVVATTGQGDFPANARSFWRSLLLKRLPGDFLDGVQFASFGLGDSSYPKFNWAARKLHKRLLQLGANEIYDAGEADQQHPEGWPIPDDVQLPPKWILKLQEGNAATAQEGHAVPGTHENLPAPEENPKLTRLDHDLRPLPDTLTATLRENKRLTPQKHWQDVRHVSLTVPEHTSYVPGDMLCITPKNFSADVDALIQMMGWEDKADQLICLVPNSHIPADDLPLPPIRGLDSYPQLTLRALLTDYLDIQAIPRRSFFAEIAHYTNDEMHKERLLEFTYTSPEYLDELWDYTTRPRRSILEVLHEFDTVKVPWQHAISVFPVLKGRQFSIASGGELKRGPEGGTKFELMIAIVKYQTVIKKIRQGVCTRYISALRPGSTLKVQLQRGGLNSSVNQLVGPTVLVGPGTGLAPLRSMLWEKAAIIKAYQEENPGVELSIGPTLLLYGGRNREADFFFEDEWQQLGQLTKLNVLTAFSRDQKQKVYVQDVIRQNYALLFKLLHDMAGSVYICGSSGQMPKAVREALTEAFQHGAEVETDRFNEQGAEQYLLGMEKTGRVPLPHNFVIMSGLRILVPVKRVIDYAVKPRVNKANTGVETAGVKHSLNPFDELSVEEAIRLRERGARNQSPMKVENILALSAGGAKCADTLRTAMAMGADRAFHVDVGESPDGGPEPLTIAKMLQGVVKSENINLVLLGKQAIDGDQGQTGQMLAGLLGWPQATQASKVDIKDADGTVEVTHEVDGGVETLRAKLPMVITTDLRLNEPRYATLPNIMKAKKKPLEKKTLADFGVEDKKRLKTLKVTEPPARQGGGKVEDVDGLIGKLKELGAL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KZ851859
EMBL· GenBank· DDBJ
RDK39998.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp