A0A370NEZ7 · A0A370NEZ7_9BURK
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsP
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids582 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 195 | Tele-phosphohistidine intermediate | |||
Binding site | 302 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 339 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 442 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 465-466 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Binding site | 466 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 476 | phosphoenolpyruvate (UniProtKB | ChEBI) | |||
Active site | 513 | Proton donor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system | |
Biological Process | phosphorylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Paraburkholderia
Accessions
- Primary accessionA0A370NEZ7
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 6-129 | Phosphotransferase system enzyme I N-terminal | |||
Domain | 161-231 | PEP-utilising enzyme mobile | |||
Domain | 258-551 | PEP-utilising enzyme C-terminal | |||
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length582
- Mass (Da)64,006
- Last updated2018-11-07 v1
- Checksum9FF06F0CA11247D8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QHKS01000002 EMBL· GenBank· DDBJ | RDK04186.1 EMBL· GenBank· DDBJ | Genomic DNA |