A0A370NEI4 · A0A370NEI4_9BURK

  • Protein
    Phosphoenolpyruvate carboxykinase [GTP]
  • Gene
    pckG
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mn2+ ion per subunit.

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site85substrate
Binding site224-226substrate
Binding site233Mn2+ (UniProtKB | ChEBI)
Binding site253Mn2+ (UniProtKB | ChEBI)
Binding site275substrate
Binding site276-281GTP (UniProtKB | ChEBI)
Active site277
Binding site302Mn2+ (UniProtKB | ChEBI)
Binding site396-398substrate
Binding site398GTP (UniProtKB | ChEBI)
Binding site429GTP (UniProtKB | ChEBI)
Binding site526-529GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionGTP binding
Molecular Functionkinase activity
Molecular Functionmanganese ion binding
Molecular Functionphosphoenolpyruvate carboxykinase (GTP) activity
Biological Processcellular response to glucose stimulus
Biological Processgluconeogenesis
Biological Processglycerol biosynthetic process from pyruvate
Biological Processphosphorylation
Biological Processpropionate catabolic process
Biological Processresponse to lipid
Biological Processresponse to starvation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoenolpyruvate carboxykinase [GTP]
  • EC number
  • Short names
    PEP carboxykinase
    ; PEPCK
  • Alternative names
    • GTP-dependent phosphoenolpyruvate carboxykinase
      (GTP-PEPCK
      )

Gene names

    • Name
      pckG
    • ORF names
      DLM46_03095

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • S27
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Paraburkholderia

Accessions

  • Primary accession
    A0A370NEI4

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain25-245Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal
Domain249-614Phosphoenolpyruvate carboxykinase C-terminal P-loop

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    618
  • Mass (Da)
    68,235
  • Last updated
    2018-11-07 v1
  • Checksum
    CFF7411077215DBE
MNHPSFEGQTTIEAPVWVKHRKLIEWVGRVAALTKPDQVVWCDGSQEEYDRLCAQMVEAGTLKRLNPAKRPNSYLAWSDPSDVARVEDRTFICSQRREDAGPTNNWIAPAEMRSTLDGLFDGAMRGRTMYVVPFSMGPLGSPIAHIGVELSDSPYVVTNMRIMTRMGRKVYDVLGEDGEFVPCVHSVGAPLAAGAKDVPWPCNSTKYIVHFPESREIWSYGSGYGGNALLGKKCFALRIASTMGRDEGWLAEHMLILGVTSPEGRKYHVAAAFPSACGKTNFAMLIPPEGLNGWKISTIGDDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTAEPPAHLIDWQRKDWTPAIAKESGRKAAHPNARFTAPASQCPSIDTEWENPAGVAIDAFIFGGRRSTTVPLVTEARNWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCGYNMSDYFGHWLKTGERLEKLNAKLPKIFCVNWFRKGAEGKFVWPGFGENMRVLSWMVGRIEGSAQGEEHAFGISPHYDDIDWSGLDFSREQFQQVISVDAAAWRDELALHAELFDTLQQGLPPALTQTKAALERKLVV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QHKS01000002
EMBL· GenBank· DDBJ
RDK03985.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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