A0A369P426 · A0A369P426_9ACTN

Function

function

The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site205Zn2+ (UniProtKB | ChEBI)
Binding site208Zn2+ (UniProtKB | ChEBI)
Binding site227Zn2+ (UniProtKB | ChEBI)
Binding site230Zn2+ (UniProtKB | ChEBI)
Active site358
Active site601Nucleophile
Binding site637substrate
Active site703

GO annotations

AspectTerm
Molecular Functionacid-amino acid ligase activity
Molecular FunctionATP binding
Molecular Functioncarbon-nitrogen ligase activity on lipid II
Molecular Functionglutaminase activity
Molecular Functionzinc ion binding
Biological Processcell wall organization
Biological Processcobalamin biosynthetic process
Biological Processglutamine metabolic process
Biological Processpeptidoglycan biosynthetic process
Biological Processregulation of cell shape

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
  • EC number
  • Alternative names
    • Lipid II isoglutaminyl synthase glutaminase subunit
      (EC:3.5.1.2
      ) . EC:3.5.1.2 (UniProtKB | ENZYME | Rhea)
  • Recommended name
    Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT

Gene names

    • Name
      murT
    • Synonyms
      gatD
    • ORF names
      C1850_03070

Organism names

Accessions

  • Primary accession
    A0A369P426

Proteomes

Interaction

Subunit

Forms a heterodimer with GatD.
Forms a heterodimer with MurT.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain59-192Mur ligase central
Domain322-436Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT C-terminal
Region465-505Disordered
Domain513-710CobB/CobQ-like glutamine amidotransferase

Sequence similarities

Belongs to the CobB/CobQ family. GatD subfamily.
Belongs to the MurCDEF family. MurT subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    756
  • Mass (Da)
    80,647
  • Last updated
    2018-11-07 v1
  • Checksum
    CF496B5E2C9CA5B3
MSLRFSLAKAAGAVSTWGLRHVARRPAANLPGKIALAIDPSLLDELRGKCAQGSVITVGTNGKTSTSNLLADAFEAAGCTIICNRTGANLAAGITSALLQQPAAQWGVFECDELWLAHVLPRLRSNYVLLLNLFRDQLDRCGEIDRIQTSIAGALAASPDTVLVYNADDPLCARIADEVPNRTVAYGLDESMGLAQNTVTDAQMCQKCDGMVRYHYRQYGQLGDYFCDQCDFARPTLDFAGRDIAIGPAGVTMEVCGPAGCESVHTPQPTPYAAYNLVASYALCREVGIPTADFQRAQDAFNPRNGRLQRYRLGGRDVLLNLAKNPTGFNQNLKIVEADQGPKMVAFFINDQVADGHDISWIWDIDFEELAGQPGTVVFAGGSRAHDLAVRLKYAGIEAAVIENIEDAFSRLGALVAAGTLPADAAVYAIANYTALPQVHAALNAMEAAEPAVAPAPTPAPIAEAAGASAAPTSCHPERSAEGAEPKDPATSEPSATPASLASEESPEPPVIIAHMLPDLLNLYGDGGNVRILEQRLRWRGIPVEVRRIQHGEAVDFSQVDLVFMGGGPDREQKLASEQLLAMRDELAVYVEGDGPLLAICGGYQILGRQWLWGDELVPGLGLIDMETRRPGTSADRLIDNMVLESPLAAHPVVGYENHAGRTYLGEGVEGFGRVVSSCGHGNNDDDRVEGARYKNVVGTYSHGPLLSKNPEVADWLLARALERRAGRTGTPAADLAPLDDGAELAANASMVKRLS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PPUT01000005
EMBL· GenBank· DDBJ
RDC45995.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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