A0A366YQZ3 · A0A366YQZ3_ECOLX

  • Protein
    Penicillin-binding protein 1B
  • Gene
    mrcB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site231Proton donor; for transglycosylase activity
Active site508Acyl-ester intermediate; for transpeptidase activity

GO annotations

AspectTerm
Cellular Componentouter membrane-bounded periplasmic space
Cellular Componentpeptidoglycan-based cell wall
Cellular Componentplasma membrane
Molecular Functionpenicillin binding
Molecular Functionpeptidoglycan glycosyltransferase activity
Molecular Functionserine-type D-Ala-D-Ala carboxypeptidase activity
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processproteolysis
Biological Processregulation of cell shape
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Penicillin-binding protein 1B
  • Short names
    PBP-1b
    ; PBP1b
  • Alternative names
    • Murein polymerase

Gene names

    • Name
      mrcB
    • ORF names
      EWK56_14790
      , G3W53_17840
      , PWL68_003251

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • SCPM-O-B-8431
    • SCPM-O-B-8431(U15)
    • 8375wB1
    • 2023QG-00028
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A366YQZ3

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane63-84Helical

Keywords

  • Cellular component

Family & Domains

Features

Showing features for compositional bias, region, domain.

Type
IDPosition(s)Description
Compositional bias1-10Basic and acidic residues
Region1-58Disordered
Domain1-77Transglycosylase PBP1b N-terminal transmembrane
Compositional bias11-25Basic residues
Compositional bias30-44Acidic residues
Compositional bias48-58Basic residues
Domain111-195Bifunctional transglycosylase second
Domain207-377Glycosyl transferase family 51
Domain471-712Penicillin-binding protein transpeptidase
Region791-842Disordered

Sequence similarities

In the C-terminal section; belongs to the transpeptidase family.
In the N-terminal section; belongs to the glycosyltransferase 51 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    842
  • Mass (Da)
    94,088
  • Last updated
    2018-11-07 v1
  • MD5 Checksum
    24CFFD9D48806A970D01AC92B8D60F1C
MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEEPMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-10Basic and acidic residues
Compositional bias11-25Basic residues
Compositional bias30-44Acidic residues
Compositional bias48-58Basic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ABKSHZ030000009
EMBL· GenBank· DDBJ
EMM9723109.1
EMBL· GenBank· DDBJ
Genomic DNA
JAAGYP010000025
EMBL· GenBank· DDBJ
NEN71968.1
EMBL· GenBank· DDBJ
Genomic DNA
SERV01000009
EMBL· GenBank· DDBJ
RYL81494.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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