A0A365PCK6 · A0A365PCK6_9ACTN
- ProteinMethionine--tRNA ligase
- GenemetG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids612 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity
- tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | methionine-tRNA ligase activity | |
Biological Process | methionyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Dietziaceae > Dietzia
Accessions
- Primary accessionA0A365PCK6
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-411 | Methionyl/Leucyl tRNA synthetase | ||||
Sequence: LTAVAWPYANGPRHIGHVSGFGVPSDVFSRYQRMAGNDVLMVSGTDEHGTPLLVQAEKEGVPVQELADRYNRVIVDDLAGLGLSYDLFTRTTTRNHYAVVQELFRGLHANGYMVAKTTTGAVSPSTGRTLPDRYIEGTCPICGDDGARGDQCDNCGNQLDPADLKNPVSKINGETPKFIETEHWFLDLPALADSLGDWLKGRKDWRPNVLKFSLNLLEDLRPRAMSRDIDWGIPIPVEGWQDRGDKKLYVWFDAVVGYLSASIEWAWRSGEPEAWRRWWTDPEAVSHYFMGKDNITFHSQIWPAELIGYDGRGAHGGEPGALGALNLPTEVVSSEFLTMSGSKFSSSRGVVIYVRDFLAEFGPDALRYFISVAGPENHDTDFTWDEYVRRTNSELVGGWGNLVNRT | ||||||
Motif | 12-22 | 'HIGH' region | ||||
Sequence: PYANGPRHIGH | ||||||
Motif | 348-352 | 'KMSKS' region | ||||
Sequence: KFSSS | ||||||
Domain | 422-527 | Methionyl-tRNA synthetase anticodon-binding | ||||
Sequence: IPRPGTLSDLDAELLREAESAFATVGAHLERSRFKAGATEAMRIVGAANRYIAATEPWKLAKEPDQRERLGTVLHTALQVVSDANTLLTPYLPSAAQQIHGAIGGQ |
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length612
- Mass (Da)67,257
- Last updated2018-11-07 v1
- Checksum01824561D5A12C70
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QNTT01000006 EMBL· GenBank· DDBJ | RBA39133.1 EMBL· GenBank· DDBJ | Genomic DNA |