A0A365P9A5 · A0A365P9A5_9ACTN

  • Protein
    Probable dual-specificity RNA methyltransferase RlmN
  • Gene
    rlmN
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site108Proton acceptor
Binding site128[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site132[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site135[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site183-184S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site217S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site240-242S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site319S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site362S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      DQ226_10640
      , R3P82_01735

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • SST1
    • IEGM 1175
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Dietziaceae > Dietzia

Accessions

  • Primary accession
    A0A365P9A5

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain114-357Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    39,788
  • Last updated
    2018-11-07 v1
  • Checksum
    CAB1AC395F919486
MAEPLPLVFDAPRRGLPPRHFADLDADGREAAMGELGVPAFRGKQLANQYFGRLEADTREMTDLPAGLREKVGEELFPSLTSSLRHVTTDEGTTRKTLWKLHDGSLVESVLMRYPDRATVCISSQAGCGMACPFCATGQGGLQRNLSAAEILEQVRVAARAMRDGEIPGGPGRLSNVVFMGMGEPLANYKRVMTAVRGIIAPPPSGFGLSQRAVTVSTVGLVPAIHKLAEEGLQVTLAVSLHTPDDELRDTLVPVNNRWPVSEVMDAARHYADATGRRVSIEYALIRDVNDQTWRGEMLGRLLAQRLGPMAHVNLIPLNPTPGSEWDASPRHQQDAFVAAVRAAGVSCTVRDTRGQEIDAACGQLAAEG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAWLKJ010000001
EMBL· GenBank· DDBJ
MDV6297826.1
EMBL· GenBank· DDBJ
Genomic DNA
QNTT01000026
EMBL· GenBank· DDBJ
RBA35267.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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