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A0A355EK59 · A0A355EK59_UNCDE

  • Protein
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • Gene
    pfp
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site13diphosphate (UniProtKB | ChEBI)
Binding site108Mg2+ (UniProtKB | ChEBI); catalytic
Site132Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site133-135substrate
Active site135Proton acceptor
Binding site179-181substrate
Binding site240substrate
Binding site300-303substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      pfp
    • ORF names
      DDZ40_03010

Organism names

Accessions

  • Primary accession
    A0A355EK59

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-326Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    417
  • Mass (Da)
    45,603
  • Last updated
    2018-11-07 v1
  • MD5 Checksum
    8F3B21DEB282D6ADC466AE7CC72C4709
MATETIGIIVGGGPAPGINGVISAATIEAINSGKKVLGIKGGFKALFEGKNDIFVPLTIDDVSRIHASGGSILRTSRDKPENAKAKFKTLMSTLKGAGIKYLITIGGDGTLYMANWIEREARGSVSVVHTPKTIDNDIPLPGGFSTFGFQTARHVGVYIVNNIIEDARTMGRWYFITTMGRHAGHLALGMGKAAGATLSIIPEEFPEEKLSFKKVADTLTGAILKRQSMGRDYGVAILAEGISEKFDLEELSSLEEVEHNEKGELRLSEIQLGRVLKDFVNKTLVDMGLEVGIVDKNIGYELRAANPIPYDVEYTRNLGYGAVRFLLKGGTGSMIVSYEGNLRPVPFVEMIDYKTGKVKIRTVDTTTETYEVARKYMIRLEREDFEGSHLAQLARTANMSPENFKARFEYVLGTPPR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DNYO01000085
EMBL· GenBank· DDBJ
HBL23066.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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