A0A351XP33 · A0A351XP33_9GAMM
- ProteinSiroheme synthase
- GenecobA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids462 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
Catalytic activity
- 2 H+ + siroheme = Fe2+ + sirohydrochlorin
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22-23 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DI | ||||||
Binding site | 43-44 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: KE | ||||||
Binding site | 225 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Active site | 248 | Proton acceptor | ||||
Sequence: D | ||||||
Active site | 270 | Proton donor | ||||
Sequence: K | ||||||
Binding site | 301-303 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: GGD | ||||||
Binding site | 306 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 331-332 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TA | ||||||
Binding site | 383 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 412 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | NAD binding | |
Molecular Function | precorrin-2 dehydrogenase activity | |
Molecular Function | sirohydrochlorin ferrochelatase activity | |
Molecular Function | uroporphyrin-III C-methyltransferase activity | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | methylation | |
Biological Process | siroheme biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSiroheme synthase
Including 3 domains:
- Recommended nameUroporphyrinogen-III C-methyltransferase
- EC number
- Short namesUrogen III methylase
- Alternative names
- Recommended namePrecorrin-2 dehydrogenase
- EC number
- Recommended nameSirohydrochlorin ferrochelatase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria
Accessions
- Primary accessionA0A351XP33
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 128 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-203 | Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase | ||||
Sequence: MNYLPIFIDITQQPCLVVGGGDIALRKINLLLKANAAVTCVSKECCNGIEKLVKDNKIIRIEKAFEATDINAQVLIVSATDDSDLNAQVSALAKTANIPVNVVDSPDLCSFVMPSIVDRSPIVIAISSAGKAPVLARLIRAKLESTIPHAYGKLAELAGNFRDQVKAKFNNIEDRRYFWEKTFSGIVAEKVFSGKVDEAKADL | ||||||
Domain | 124-145 | Siroheme synthase central | ||||
Sequence: IAISSAGKAPVLARLIRAKLES | ||||||
Domain | 150-207 | Sirohaem synthase dimerisation | ||||
Sequence: AYGKLAELAGNFRDQVKAKFNNIEDRRYFWEKTFSGIVAEKVFSGKVDEAKADLQAQL | ||||||
Region | 216-462 | Uroporphyrinogen-III C-methyltransferase | ||||
Sequence: GEVYLVGGGPGDPDLLTFKALRLMQQADVILYDRLVSDGVMELVRRDAELIYVGKERDNHAVPQGDINQLLVDLAKQGRRVCRLKGGDPFIFGRGGEEIETLAENNIPFQVVPGITAASGCSAYSGIPLTHRDYSQSCRFVTGHLKDGSMNLPWDELAVEQQTIVFYMALVGARHLSEQLISHGMRGDMPVALVEKGTTPDHQVYVTTLAELPNLVENTTIHAPTLIIIGEVVKLREKLNWFDADND | ||||||
Domain | 218-427 | Tetrapyrrole methylase | ||||
Sequence: VYLVGGGPGDPDLLTFKALRLMQQADVILYDRLVSDGVMELVRRDAELIYVGKERDNHAVPQGDINQLLVDLAKQGRRVCRLKGGDPFIFGRGGEEIETLAENNIPFQVVPGITAASGCSAYSGIPLTHRDYSQSCRFVTGHLKDGSMNLPWDELAVEQQTIVFYMALVGARHLSEQLISHGMRGDMPVALVEKGTTPDHQVYVTTLAEL |
Sequence similarities
Belongs to the precorrin methyltransferase family.
In the C-terminal section; belongs to the precorrin methyltransferase family.
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length462
- Mass (Da)50,397
- Last updated2018-11-07 v1
- Checksum82D08BA0DC3DB415
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DMBH01000028 EMBL· GenBank· DDBJ | HAD99074.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMBK01000009 EMBL· GenBank· DDBJ | HAE70079.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMDS01000008 EMBL· GenBank· DDBJ | HAG47336.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMQZ01000024 EMBL· GenBank· DDBJ | HAO44475.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMRA01000124 EMBL· GenBank· DDBJ | HAO53896.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMRD01000050 EMBL· GenBank· DDBJ | HAO70285.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMRG01000076 EMBL· GenBank· DDBJ | HAO97890.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMRF01000064 EMBL· GenBank· DDBJ | HAP05563.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMRE01000001 EMBL· GenBank· DDBJ | HAP44802.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DMJS01000116 EMBL· GenBank· DDBJ | HAP92831.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DNRK01000101 EMBL· GenBank· DDBJ | HBG03696.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DOBR01000126 EMBL· GenBank· DDBJ | HBN58491.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DOWW01000119 EMBL· GenBank· DDBJ | HCA68786.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DPFW01000094 EMBL· GenBank· DDBJ | HCH58704.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DPMG01000119 EMBL· GenBank· DDBJ | HCV74928.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DPPC01000045 EMBL· GenBank· DDBJ | HCW71725.1 EMBL· GenBank· DDBJ | Genomic DNA |