A0A350LZP5 · A0A350LZP5_UNCXX
- ProteinDNA ligase
- GeneligA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids657 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 30-34 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 79-80 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 109 | NAD+ (UniProtKB | ChEBI) | |||
Active site | 111 | N6-AMP-lysine intermediate | |||
Binding site | 132 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 168 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 283 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 307 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 399 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 402 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 422 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | DNA ligase (NAD+) activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair, DNA ligation | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Sequence
- Sequence statusComplete
- Length657
- Mass (Da)74,448
- Last updated2018-11-07 v1
- ChecksumF7523AD0D6F0F165
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DNCM01000099 EMBL· GenBank· DDBJ | HAW50012.1 EMBL· GenBank· DDBJ | Genomic DNA |