A0A346AFT9 · A0A346AFT9_9BACT
- ProteinRNA 3'-terminal phosphate cyclase
- GenertcA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids350 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 100 | ATP (UniProtKB | ChEBI) | |||
Binding site | 286-290 | ATP (UniProtKB | ChEBI) | |||
Active site | 311 | Tele-AMP-histidine intermediate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | RNA-3'-phosphate cyclase activity | |
Biological Process | RNA processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA 3'-terminal phosphate cyclase
- EC number
- Short namesRNA cyclase ; RNA-3'-phosphate cyclase
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > environmental samples
Accessions
- Primary accessionA0A346AFT9
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 9-329 | RNA 3'-terminal phosphate cyclase | |||
Domain | 182-277 | RNA 3'-terminal phosphate cyclase insert | |||
Sequence similarities
Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length350
- Mass (Da)37,622
- Last updated2018-11-07 v1
- ChecksumC93F7C840404553F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MH480551 EMBL· GenBank· DDBJ | AXL06101.1 EMBL· GenBank· DDBJ | Genomic DNA |