A0A345AH88 · A0A345AH88_9HEPC
- ProteinGenome polyprotein
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids3016 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Cysteine protease required for the proteolytic auto-cleavage between the non-structural proteins NS2 and NS3. The N-terminus of NS3 is required for the function of NS2 protease (active region NS2-3). Promotes the initiation of viral particle assembly by mediating the interaction between structural and non-structural proteins.
Induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex. NS4B self-interaction contributes to its function in membranous web formation. Promotes host TRIF protein degradation in a CASP8-dependent manner thereby inhibiting host TLR3-mediated interferon signaling. Disrupts the interaction between STING and TBK1 contributing to the inhibition of interferon signaling.
RNA-dependent RNA polymerase that performs primer-template recognition and RNA synthesis during viral replication. Initiates RNA transcription/replication at a flavin adenine dinucleotide (FAD), resulting in a 5'- FAD cap on viral RNAs. In this way, recognition of viral 5' RNA by host pattern recognition receptors can be bypassed, thereby evading activation of antiviral pathways.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
Cofactor
Protein has several cofactor binding sites:
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGenome polyprotein
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Flasuviricetes > Amarillovirales > Flaviviridae > Hepacivirus
Accessions
- Primary accessionA0A345AH88
Subcellular Location
UniProt Annotation
GO Annotation
Host endoplasmic reticulum membrane ; Multi-pass membrane protein
Host endoplasmic reticulum membrane ; Peripheral membrane protein
Host endoplasmic reticulum membrane ; Single-pass type I membrane protein
Virion membrane ; Single-pass type I membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 264-287 | Helical | |||
Transmembrane | 368-387 | Helical | |||
Transmembrane | 722-743 | Helical | |||
Transmembrane | 755-782 | Helical | |||
Transmembrane | 789-807 | Helical | |||
Transmembrane | 819-839 | Helical | |||
Transmembrane | 877-905 | Helical | |||
Transmembrane | 1664-1687 | Helical | |||
Transmembrane | 1831-1849 | Helical | |||
Transmembrane | 1855-1874 | Helical | |||
Transmembrane | 1886-1906 | Helical | |||
Transmembrane | 2837-2858 | Helical | |||
Transmembrane | 2996-3013 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Homohexamer. Homoheptamer. Interacts with protease NS2.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-78 | Disordered | |||
Compositional bias | 47-61 | Basic and acidic residues | |||
Domain | 907-1030 | Peptidase C18 | |||
Domain | 1031-1212 | Peptidase S29 | |||
Domain | 1221-1373 | Helicase ATP-binding | |||
Domain | 1365-1542 | Helicase C-terminal | |||
Region | 2314-2339 | Disordered | |||
Compositional bias | 2315-2332 | Pro residues | |||
Region | 2354-2414 | Disordered | |||
Domain | 2639-2757 | RdRp catalytic | |||
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length3,016
- Mass (Da)327,846
- Last updated2018-11-07 v1
- Checksum5C2AA5A00F67F2BE
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 47-61 | Basic and acidic residues | |||
Compositional bias | 2315-2332 | Pro residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MH590698 EMBL· GenBank· DDBJ | AXF35722.1 EMBL· GenBank· DDBJ | Genomic RNA |