A0A345AH88 · A0A345AH88_9HEPC

Function

function

Cysteine protease required for the proteolytic auto-cleavage between the non-structural proteins NS2 and NS3. The N-terminus of NS3 is required for the function of NS2 protease (active region NS2-3). Promotes the initiation of viral particle assembly by mediating the interaction between structural and non-structural proteins.
Induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex. NS4B self-interaction contributes to its function in membranous web formation. Promotes host TRIF protein degradation in a CASP8-dependent manner thereby inhibiting host TLR3-mediated interferon signaling. Disrupts the interaction between STING and TBK1 contributing to the inhibition of interferon signaling.
RNA-dependent RNA polymerase that performs primer-template recognition and RNA synthesis during viral replication. Initiates RNA transcription/replication at a flavin adenine dinucleotide (FAD), resulting in a 5'- FAD cap on viral RNAs. In this way, recognition of viral 5' RNA by host pattern recognition receptors can be bypassed, thereby evading activation of antiviral pathways.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell lipid droplet
Cellular Componenthost cell mitochondrion
Cellular Componenthost cell nucleus
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componenthost cell plasma membrane
Cellular Componentlipid droplet
Cellular Componentplasma membrane
Cellular Componentribonucleoprotein complex
Cellular Componentviral envelope
Cellular Componentviral nucleocapsid
Cellular Componentvirion membrane
Molecular FunctionATP binding
Molecular Functionchannel activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionribonucleoside triphosphate phosphatase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionserine-type endopeptidase activity
Molecular Functionstructural molecule activity
Molecular Functionzinc ion binding
Biological Processapoptotic process
Biological Processfusion of virus membrane with host endosome membrane
Biological Processinduction by virus of host autophagy
Biological Processmonoatomic ion transmembrane transport
Biological Processproteolysis
Biological Processsymbiont entry into host cell
Biological Processsymbiont-mediated perturbation of host cell cycle G1/S transition checkpoint
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity
Biological Processsymbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity
Biological Processsymbiont-mediated suppression of host TRAF-mediated signal transduction
Biological Processsymbiont-mediated suppression of host type I interferon-mediated signaling pathway
Biological Processtransformation of host cell by virus
Biological Processviral RNA genome replication
Biological Processvirion attachment to host cell
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Genome polyprotein

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GT8-1
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Kitrinoviricota > Flasuviricetes > Amarillovirales > Flaviviridae > Hepacivirus

Accessions

  • Primary accession
    A0A345AH88

Subcellular Location

Host cell membrane
Host endoplasmic reticulum membrane
; Multi-pass membrane protein
Host endoplasmic reticulum membrane
; Peripheral membrane protein
Host endoplasmic reticulum membrane
; Single-pass type I membrane protein
Host lipid droplet
Host mitochondrion
Host nucleus
Virion membrane
; Single-pass type I membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane264-287Helical
Transmembrane368-387Helical
Transmembrane722-743Helical
Transmembrane755-782Helical
Transmembrane789-807Helical
Transmembrane819-839Helical
Transmembrane877-905Helical
Transmembrane1664-1687Helical
Transmembrane1831-1849Helical
Transmembrane1855-1874Helical
Transmembrane1886-1906Helical
Transmembrane2837-2858Helical
Transmembrane2996-3013Helical

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homohexamer. Homoheptamer. Interacts with protease NS2.

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region1-78Disordered
Compositional bias47-61Basic and acidic residues
Domain907-1030Peptidase C18
Domain1031-1212Peptidase S29
Domain1221-1373Helicase ATP-binding
Domain1365-1542Helicase C-terminal
Region2314-2339Disordered
Compositional bias2315-2332Pro residues
Region2354-2414Disordered
Domain2639-2757RdRp catalytic

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    3,016
  • Mass (Da)
    327,846
  • Last updated
    2018-11-07 v1
  • Checksum
    5C2AA5A00F67F2BE
MSTLPKPQRKTKRNTIRRPQDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKVRQPRGRTWGQPGYPWPLYGNEGCGWAGWLLSPRGSRPNWGPNDPRRRSRNLGKVIDTLTCGFADLMGYIPVLGGPLGGVAAALAHGVRAVEDGVNYATGNLPGCSFSIFLLALLSCLTVPASAYQVRNESGLYIVTNDCANSSIIFEGEDVILHTPGCVPCVRTGNVSSCWAGVTPTLSVRDPGTPTAGFRRHVDLIAGAAVVCSGLYIGDICGGVFLVGQLFTFQPRKYWTTQDCNCSIFPGHVTGHRMAWDMMQNWSPTVTWVISSLLRIPQLLFDMVSGGHWGVLAAIAFYGMSSNWLKVLVVMVLFAGVDGETMLTASRSAQTTRAFTGLFSVGSSQKLRLVNTNGSWHINSTALNCNDSLNTGFIAALFYTRAHLNLTGCPERMSACRRITDFAQGWGPLTYANISGPQEDKPYCWHYPPRPCGIVPARDVCGPVYCFTPSPVVVGTTDRRGQATFNWGERADDVLILNSTRPPSGGWFGCTWMNATGFTKTCGAPPCRTRPLGDDNNTLVCPTDCFRKHPEATYSRCGSGPWLTPRCLVDYPYRLWHYPCTVNYTIHKIRLYIGGTEHRLDAACNWTRGEKCNLDDRDRADLSPLLFSTTELAILPCSFTPMPALSTGLIHLHQNIVDVQYLYGIGSIIVSWAIKWEYVVLAFLMLADSRICLCLWLMFLVGQAEAALENLIALNTISAAGTHGLLFSLLAICAMWYIKGRAVPAFTYCYFQIWPMLLLILALPERARAMDNHVAGTLGAGVIALVTLATLLPTYKLFLAKVLWWIQYFIAHAEARLQQWVPPLRVRGGRDSIILLTCILHPSLGFEVTKCLLALMGPVYILQQSLLKVPYFIRAHGLLRACMLVRNMAGGKYAQMLLIRLGSWTGTYLYDHLSPLKDWASEGLRDLAVAVEPVIFTPMEKKIITWGADSVACGDILSGLPVSARRGHEVLLGPADDIRGGGWRLLSPITAYAQQTRGLVGTIVTSLTGRDKNTVEGEVQVLSTATQSFLGTTINGVLWTVYHGAGSKTLAAPKGPVIQMYTNVDKDLVGWPAPPGAKSLTPCCCGASDLYLVTRNADVIPVRRQGDTRGALLSPRPLATLKGSSGGPLLCSAGHVVGLFRAAVVTRGVAKAVDFIPVENLETTARSPVFSDNSAPPAVPQTYQVGYLHAPTGSGKSTKVPAAYAAQGYRVLVLNPSVAATLGFGAYMSKAHGIDPNIRTGVRTVTTGGSITYSTYGKFLADGGCSGGAYDIIICDECHAIDATSILGIGTVLDQAETAGVRLVVLATATPPGSITVPHPNIEEVGLGHEGEIPFYGKAIPLSQVKGGRHLIFCHSKKKCDELAKQLTSLGVNAVAYYRGLDVSVIPQTGDVVVCATDALMTGFTGDFDSVIDCNVAVTQVVDFSLDPTFSIETTTVPQDAVSRSQRRGRTGRGKHGIYRFVSQGERPSGMFDSVVLCEAYDTGASWYELTPAETTVRLRAYLSTPGLPVCPDHLEFWEGVFTGLTHIDAHFLSQTKQSGEAFSYLVAYQATVCARAKAPPPSWDQMWKCLTRLKPTLVGPTPLLYRLGAVSNEITLTHPVTKYIMACMSADLEVVTSTWVLLGGLLAALAAYCLSVGCVVVIGRITLSGKPAVIPDREVLYQQFDEMEECSLYTPYIERGRDIAEQFRQKAIGILQNTTQRAEDLKPAVEAAWPRLEQFWGKHMWNFVSGIQYLAGLSTLPGNPAIASLMSFSASLTSPFQTSTTLLLNIMGGWVASQLASPTGSTAFVVSGIAGAAVGSIGLGKVIVDVLAGYGAGVAGALVAFKIMSGECPSTEDMVNLLPAILSPGALVVGVVCAAILRRHVGPQEGAVQWMNRLIAFASRGNHVSPTHYVTDTDAAAKVNAILSSLTITSLLRKLHQWITDDYATPCGNSWLSDVWDWVCTVLSDFKSWLKARLLPSLPGVPYLSCQVGFRGVWRGDGINTTTCPCGAVITGHVKNGTMRIVGPKLCSNTWRNTFPINSSTTGPSVPVPAPNYKKAMWRVSAEEYVEITQFRQSHYVTGQTADNLKCPCQVPSPEFFSFVDGVRIHRYLPDVKPLLRDEVSFSVGLNSYVVGSQLPCEPEPDVAALTSMLRDPDHITAESAARRLARGSPPSLASSSASQLSAPSLKATCDTHRPHPDIDLIEANLLWRQEMGGNITRVESENKVVILDSFDPLVAGEDDREISVSADCFKKHYDFPAALPIWARPDYNPPLLESWKKPDYEAPQVAGCPLPPPKPLPIPPPRRRRVVQLDQSNVASALALLAEKSFPPSSGDGTSGLGSGSLPEPEVPESCASTKDSDAESYSSMPPLEGEPGDPDLDSGSWDTVSEESDSVVCCSMSYSWTGALVTPCGPEEEKLPINPLSNSLIRHHNLVYSTSSKSAGLRQKKVTFDRLQVLDSHYDDVLKEVKLRASKVKVDTLSVEEACRLTPSHSARSKFGYGAKEVRGLDRKAINHINSVWEDLLSDNSEPIPTTIMAKNEVFCVNPAKGGRKAARLIVYPDLGVRVCEKRALYDMTKKLPIAIMGGAYGFQYSPQQRVDYLLKAWRSKKTPMGFSYDTRCFDSTVTERDIRTELDIYMQASMDSETRTVVKALTERLYCGGPMFNSKGQTCGYRRCRASGVLPTSMANTITCYLKASAAARAAGLKSPTMLVCGDDLVVISESLGVGEDAAALRAFTEAMTRYSAPPGDPPQPEYDLERITSCSSNVSVAHDGLGKRYYYLTRDPVTPLARAAWETARHTPVNSWLGNIILYAPTIWVRMVLMTHFFGILQSREELEKAMDFDMYGVTYSVTPLDLPQIIERLHGLAAFSLHGYSPGELTRVAGALRKLGAPPLRAWRHRARAVRARLIAQGGRAATCGHYLFNWAVKTKLKHXXXXXXXXXXXXXXXXXXXXXGDVYHSASRARPRYLLLCLLLLSVGVGLVLLPNR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias47-61Basic and acidic residues
Compositional bias2315-2332Pro residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MH590698
EMBL· GenBank· DDBJ
AXF35722.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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