A0A328WGZ0 · A0A328WGZ0_PAELA

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site593ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      DP091_04350

Organism names

Accessions

  • Primary accession
    A0A328WGZ0

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain17-621Aminoacyl-tRNA synthetase class Ia
Motif47-57'HIGH' region
Motif590-594'KMSKS' region
Domain676-821Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,029
  • Mass (Da)
    116,112
  • Last updated
    2018-10-10 v1
  • Checksum
    073D03DD8978EE3E
MNRVDVKEKARARELRILNKWNEENTFKKSIELRAGKPNYVFYEGPPTANGAPHIGHVLGRVIKDFVGRYQTMKGYHVVRKAGWDTHGLPVELGVEKQLGISGKQEIENYGVEAFIKKCKASVFEYEHKWRELTEAIAYWTDLDHPYITLENNYIESVWNILATIHDKGLLYRGHRVSPYCPCCQTTLSSHEVAQGYEDVKDLSATAKFKLHDSGEYVLAWTTTPWTLPSHVALAVNPDMDYARVEQEDGIYIVAKNLVEDVMKGDFTVLSTLKGSELVGKTYDPPFPYVKAEKANLIVGASFVTDASGTGIVHMAPAHGEDDYRVCRENGISFVSVVNGQGRYTDEVTDFAGRFVKDCDLDIVKALSEKGLLYSKEKYEHSYPFCWRCKSPLLYYAMESWFIETTAVKDQLIANNNEVTWYPGHVREGRFGKFLEDLVDWNISRNRYWGTPLNVWVCDSCEGQFSPHSIAELRSNAVGEVAEDLELHKPYVDEVKVKCPHCEGGVMERTSEVIDVWFDSGSMPYAQYHYPFEDKEKFEQQYPADMICEGIDQTRGWFYSLLAVSTLFTGKAPYKAVMATGHILDENGQKMSKSKGNVIDPWEIIEEYGTDAFRWALLADSAPWNSKRFSKGIVGEAKSKVVDTLVNTHAFLTLYAGIDGYDPKEHHFQGSNHKLDRWILSRLNSLIQVVDKGLAVNDFLNAAKAIEAYVDELSNWYIRRSRDRFWGSGLGEDKLAAYGTLTHVLLTLSKLMAPFTPMLSEDIFVNLGGGESVHLADFPQADEALIDKALEQDMETARQIVELARNVRNETGIKTRQPLSELIVSMDREFDLAGYEDIIKDEINVKAIEVETSDSGFVDFMLKMNLKVAGKKYGKNIGFLQGFLKGMSSDETRKVVQTGGVNVTSPEGEELQITSEELLVEKKAKEGFASASGYGLTVALNTDITRELEQEGWVREVVRAVQDTRKKLDLPIEKRVNLILDTDEELKVAITAFEDVLRENVLVNDLQFGQSEDMERVELGGKTIGIHIA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QLYT01000008
EMBL· GenBank· DDBJ
RAR45523.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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