A0A328UEJ3 · A0A328UEJ3_9FIRM
- ProteinMethionine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids786 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.
Catalytic activity
- (6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine
Cofactor
Protein has several cofactor binding sites:
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 201 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 264 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 265 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | cobalamin binding | |
Molecular Function | metal ion binding | |
Molecular Function | methionine synthase activity | |
Biological Process | homocysteine metabolic process | |
Biological Process | methylation | |
Biological Process | tetrahydrofolate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Acutalibacteraceae > Hydrogeniiclostridium
Accessions
- Primary accessionA0A328UEJ3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-279 | Hcy-binding | |||
Domain | 307-551 | Pterin-binding | |||
Domain | 571-664 | B12-binding N-terminal | |||
Domain | 667-786 | B12-binding | |||
Sequence similarities
Belongs to the vitamin-B12 dependent methionine synthase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length786
- Mass (Da)84,426
- Last updated2018-10-10 v1
- MD5 Checksum50B1C1F83C398A81C88301A29C47B66C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QLYR01000001 EMBL· GenBank· DDBJ | RAQ30297.1 EMBL· GenBank· DDBJ | Genomic DNA |