A0A328U8C9 · A0A328U8C9_9FIRM
- ProteinReplicative DNA helicase
- GenednaB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids458 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
The main replicative DNA helicase, it participates in initiation and elongation during chromosome replication. Travels ahead of the DNA replisome, separating dsDNA into templates for DNA synthesis. A processive ATP-dependent 5'-3' DNA helicase it has DNA-dependent ATPase activity.
Catalytic activity
- ATP + H2O = ADP + phosphate + H+
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | primosome complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Biological Process | DNA replication, synthesis of primer | |
Biological Process | DNA unwinding involved in DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicative DNA helicase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Acutalibacteraceae > Hydrogeniiclostridium
Accessions
- Primary accessionA0A328U8C9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Structure
Sequence
- Sequence statusComplete
- Length458
- Mass (Da)51,079
- Last updated2018-10-10 v1
- MD5 Checksum17A283549FDA1C5F639366F292A85093
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QLYR01000015 EMBL· GenBank· DDBJ | RAQ22163.1 EMBL· GenBank· DDBJ | Genomic DNA |